ArticlePharmacology, biochemistry, and behavior2015
Prototypical anxiolytics do not reduce anxiety-like behavior in the open field in C57BL/6J mice.
Article in Pharmacology, biochemistry, and behavior, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Under or Absent Reporting of Light Stimuli in Testing of Anxiety-Like Behaviors in Rodents: The Need for Standardization.Frontiers in molecular neuroscience · 2022Pooled it
- G9a inhibition reduces stress-potentiated alcohol drinking in both male and female mice: Implications for safe and effective pharmacological therapeutics for alcohol use disorder.Alcohol, clinical & experimental research · 2026Article
- Anxiety-like behaviour in mice after mild repetitive head impacts during the subacute phase.Frontiers in behavioral neuroscience · 2026Article
- Persistent priming of hypothalamic microglia is associated with sensitization of the hypothalamic-pituitary-adrenal axis to acute stress, hyperactivity and behavioral response disruption in male rats.Frontiers in immunology · 2026Article
- Ventral subiculum control of avoidance behavior and hypothalamic-pituitary-adrenal axis reactivity via the bed nucleus of the stria terminalis in male and female mice - ISPNE 2024 Dirk Helhammer Award.Psychoneuroendocrinology · 2025Article
- Testing associations between human anxiety and genes previously implicated by mouse anxiety models.Genes, brain, and behavior · 2023Article
- Anxiolytic-Pharmaceuticals (Basel, Switzerland) · 2023Article
- Neuroligin-3 in dopaminergic circuits promotes behavioural and neurobiological adaptations to chronic morphine exposure.Addiction biology · 2023Article
- Thigmotaxis in a virtual human open field test.Scientific reports · 2021Article
- Measuring Anxiety-Like Behaviors in Rodent Models of Traumatic Brain Injury.Frontiers in behavioral neuroscience · 2021Review
- FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice.Frontiers in pharmacology · 2021Article
- The cholinesterase inhibitor donepezil has antidepressant-like properties in the mouse forced swim test.Translational psychiatry · 2020Article
- Divergent behavioral responses in protracted opioid withdrawal in male and female C57BL/6J mice.The European journal of neuroscience · 2020Article
- Adolescent chronic variable social stress influences exploratory behavior and nicotine responses in male, but not female, BALB/cJ mice.Brain research bulletin · 2018Article
- Exposure to chronic variable social stress during adolescence alters affect-related behaviors and adrenocortical activity in adult male and female inbred mice.Developmental psychobiology · 2017Article
- Blockade of the 5-HT transporter contributes to the behavioural, neuronal and molecular effects of cocaine.British journal of pharmacology · 2017Article
- Neuropsychiatric Symptom Modeling in Male and Female C57BL/6J Mice after Experimental Traumatic Brain Injury.Journal of neurotrauma · 2017Article
- NPAS2 Regulation of Anxiety-Like Behavior and GABAA Receptors.Frontiers in molecular neuroscience · 2017Article
- Ovariectomy results in inbred strain-specific increases in anxiety-like behavior in mice.Physiology & behavior · 2016Article
- Preclinical animal anxiety research - flaws and prejudices.Pharmacology research & perspectives · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Understanding and effectively treating anxiety disorders are a challenge for both scientists and clinicians. Despite a variety of available therapies, the efficacy of current treatments is still not optimal and adverse side effects can result in non-compliance. Animal models have been useful for studying the underlying biology of anxiety and assessing the anxiolytic properties of potential therapeutics. The open field (OF) is a commonly used assay of anxiety-like behavior. The OF was developed and validated in rats and then transferred to use in the mouse with only limited validation. The present study tests the efficacy of prototypical benzodiazepine anxiolytics, chlordiazepoxide (CDP) and diazepam (DZ), for increasing center time in the OF in C57BL/6J (B6) mice. Multiple doses of CDP and DZ did not change time spent in the center of the OF. Increasing illumination in the OF did not alter these results. The non-benzodiazepine anxiolytic, buspirone (BUSP) also failed to increase center time in the OF while the anxiogenic meta-chlorophenylpiperazine (mCPP) increased center time. Additional inbred mouse strains, BALB/cJ (BALB) and DBA/2J (D2) did not show any change in center time in response to CDP. Moreover, evaluation of CDP in B6 mice in the elevated plus maze (EPM), elevated zero maze (EZM) and light dark assay (LD) did not reveal changes in anxiety-like behavior while stress-induced hyperthermia (SIH) was decreased by DZ. Pharmacokinetic (PK) studies suggest that adequate CDP is present to induce anxiolysis. We conclude that the measure of center time in the OF does not show predictive validity for anxiolysis in these inbred mouse strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.