ArticleNature protocols2015
Multiplexed locus-specific analysis of DNA methylation in single cells.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Advances in Epigenomic Sequencing and Their Applications in Cancer Diagnostics.Precision chemistry · 2025Review
- Nanoimprinted Plasmonic Crystals for Cost-Effective SERS Identification of Methylated DNAs.Sensors (Basel, Switzerland) · 2024Article
- Beyond single cells: microfluidics empowering multiomics analysis.Analytical and bioanalytical chemistry · 2024Review
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
- Deciphering functional tumor states at single-cell resolution.The EMBO journal · 2022Review
- scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells.Genome biology · 2022Article
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Variable allelic expression of imprinted genes at theFrontiers in cell and developmental biology · 2022Article
- Advances in measuring DNA methylation.Blood science (Baltimore, Md.) · 2022Review
- Biophysics is reshaping our perception of the epigenome: from DNA-level to high-throughput studies.Biophysical reports · 2021Review
- Development of a multiplex methylation-sensitive restriction enzyme-based SNP typing system for deconvolution of semen-containing mixtures.International journal of legal medicine · 2021Article
- DNA methylation patterns expose variations in enhancer-chromatin modifications during embryonic stem cell differentiation.PLoS genetics · 2021Article
- Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.Communications biology · 2021Article
- DNA methylation studies in cattle.Journal of applied genetics · 2021Review
- The role of ZFP57 and additional KRAB-zinc finger proteins in the maintenance of human imprinted methylation and multi-locus imprinting disturbances.Nucleic acids research · 2020Article
- A novel fluorescent biosensor based on dendritic DNA nanostructure in combination with ligase reaction for ultrasensitive detection of DNA methylation.Journal of nanobiotechnology · 2019Article
- Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs.Molecular cell · 2019Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- A 20-Gene Set Predictive of Progression to Severe Dengue.Cell reports · 2019Article
- Epigenetics and bone diseases.Genetics research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol details a method for measuring the DNA methylation state of multiple target sites in single cells, otherwise known as single-cell restriction analysis of methylation (SCRAM). The basic steps include isolating and lysing single cells, digesting genomic DNA with a methylation-sensitive restriction endonuclease (MSRE) and amplification of multiple targets by two rounds of PCR to determine the methylation status of target sites. The method can reliably and accurately detect the methylation status of multiple target sites in each single cell, and it can be completed in a relatively short time (<2 d) at low cost. Consequently, the method may be preferable over whole-genome methods in applications requiring highly reliable and cost-effective coverage of specific target sites in all cells from a sample and in cases when the DNA methylation states of single CpG sites are representative of the methylation status of corresponding regions of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.