Evidence map›Paper›PMID 25811766›Full record

ArticleInternational journal of environmental research and public health2015

Isolation and molecular characterization of free-living amoebae from different water sources in Italy.

Margherita Montalbano Di Filippo, Maristella Santoro, Piero Lovreglio, Rosa Monno, Carmen Capolongo, Carla Calia, Luciana Fumarola, Rossella D'Alfonso, Federica Berrilli, David Di Cave

Open access · goldAbstract read
In one paragraph

Article in International journal of environmental research and public health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
4.3field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 54 citations in OpenAlex.

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  8. Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines.International journal of environmental research and public health · 2019
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  9. ThermotolerantJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019
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  10. Article
  11. Review
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  13. MethodsX · 2018
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  14. Article
  15. World journal of gastroenterology · 2017
    Article
  16. Article
  17. Isolation and Molecular Identification ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Margherita Montalbano Di FilippoDepartment of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. montalbano.margherita89@gmail.com.
Maristella SantoroDepartment of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. maristella5384@gmail.com.
Piero LovreglioInterdisciplinary Department of Medicine, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. piero.lovreglio@uniba.it.
Rosa MonnoDepartment of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. rosa.monno@uniba.it.
Carmen CapolongoDepartment of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. carla.calia@virgilio.it.
Carla CaliaDepartment of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. carla.calia@virgilio.it.
Luciana FumarolaDepartment of Basic Medical Science, Neuroscience and Sense Organ, University of Bari, Piazza G. Cesare 11, 70124 Bari, Italy. luciana.fumarola@uniba.it.
Rossella D'AlfonsoDepartment of Systems Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. dalfonso@uniroma2.it.
Federica BerrilliDepartment of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. berrilli@uniroma2.it.
David Di CaveDepartment of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. dicave@uniroma2.it.
University of Bari Aldo Moro · ITUniversity of Rome Tor Vergata · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Free-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates. Other FLA species, including Vahlkampfia sp. and Naegleria spp., previously reported in Italy, were not recovered. The occurrence of potentially pathogenic free-living amoebae in habitats related to human population, as reported in the present study, supports the relevance of FLA as a potential health threat to humans.

Indexed as

Water MicrobiologyAmoebozoaDNA, ProtozoanDNA, RibosomalGenotypeItalyDNA, ProtozoanDNA, Ribosomal

Identifiers

PMID25811766
PMCPMC4410193
OpenAlexW2017777057

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.