Evidence map›Paper›PMID 25811625›Full record

ArticlePloS one2015

Biochanin a gastroprotective effects in ethanol-induced gastric mucosal ulceration in rats.

Maryam Hajrezaie, NurAin Salehen, Hamed Karimian, Maryam Zahedifard, Keivan Shams, Rami Al Batran, Nazia Abdul Majid, Shaden A M Khalifa, Hapipah Mohd Ali, Hesham El-Seedi and 1 more

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 32 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed, 1 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.

  1. Pooled it
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  5. Article
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  13. Potential Active Constituents fromPharmaceuticals (Basel, Switzerland) · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Maryam HajrezaieDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
NurAin SalehenDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Hamed KarimianDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Maryam ZahedifardDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Keivan ShamsDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Rami Al BatranDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Nazia Abdul MajidInstitute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Shaden A M KhalifaDepartment of Experimental Hematology, Karolinska University Hospital, SE-141 86, Stockholm, Sweden.
Hapipah Mohd AliDepartment of Chemistry, University of Malaya, Kuala Lumpur, Malaysia.
Hesham El-SeediDepartment of Experimental Hematology, Karolinska University Hospital, SE-141 86, Stockholm, Sweden; Division of Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, SE-75 123, Uppsala, Sweden.
Mahmood Ameen AbdullaDepartment of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
University of Malaya · MYKarolinska University Hospital · SEUppsala University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBiochanin A notable bioactive compound which is found in so many traditional medicinal plant. In vivo study was conducted to assess the protective effect of biochanin A on the gastric wall of Spraguedawley rats` stomachs. METHODOLOGY: The experimental set included different animal groups. Specifically, four groups with gastric mucosal lesions were receiving either a) Ulcer control group treated with absolute ethanol (5 ml/kg), b) 20 mg/kg of omeprazole as reference group, c) 25 of biochanin A, d) 50 mg/kg of biochanin A. Histopathological sectioning followed by immunohistochemistry staining were undertaken to evaluate the influence of the different treatments on gastric wall mucosal layer. The gastric secretions were collected in the form of homogenate and exposed to superoxide dismutase (SOD) and nitric oxide enzyme (NO) and the level of malondialdehyde (MDA) and protein content were measured. Ulceration and patchy haemorrhage were clearly observed by light microscopy. The morphology of the gastric wall as confirmed by immunohistochemistry and fluorescent microscopic observations, exhibited sever deformity with notable thickness, oedematous and complete loss of the mucosal coverage however the biochanin-pretreated animals, similar to the omeprazole-pretreated animals, showed less damage compared to the ulcer control group. Moreover, up-regulation of Hsp70 protein and down-regulation of Bax protein were detected in the biochanin A pre-treated groups and the gastric glandular mucosa was positively stained with Periodic Acid Schiff (PAS) staining and the Leucocytes infiltration was commonly seen. Biochanin A displayed a great increase in SOD and NO levels and decreased the release of MDA.

conclusionsThis gastroprotective effect of biochanin A could be attributed to the enhancement of cellular metabolic cycles perceived as an increase in the SOD, NO activity, and decrease in the level of MDA, and also decrease in level of Bax expression and increase the Hsp70 expression level.

Indexed as

AnimalsAntioxidantsbcl-2-Associated X ProteinEthanolFemaleGastric MucosaGenisteinHSP70 Heat-Shock ProteinsImmunohistochemistryLiver Function TestsMalondialdehydeNitric OxideProtective AgentsRats, Sprague-DawleyStaining and LabelingStomach UlcerAntioxidantsbcl-2-Associated X Proteinbiochanin AEthanolGenisteinHSP70 Heat-Shock ProteinsMalondialdehydeNitric OxideProtective AgentsSuperoxide Dismutase

Identifiers

PMID25811625
PMCPMC4374864
OpenAlexW2059348329

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.