ArticlePloS one2015
Evolutionarily conserved 5'-3' exoribonuclease Xrn1 accumulates at plasma membrane-associated eisosomes in post-diauxic yeast.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- The Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress inmSphere · 2024Article
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- The joint action of yeast eisosomes and membraneless organelles in response to ethanol stress.Heliyon · 2024Article
- Live cell fluorescence microscopy-an end-to-end workflow for high-throughput image and data analysis.Biology methods & protocols · 2024Review
- Xrn1 biochemically associates with eisosome proteins after the post diauxic shift in yeast.microPublication biology · 2023Article
- Decoupling of degradation from deadenylation reshapes poly(A) tail length in yeast meiosis.Nature structural & molecular biology · 2021Article
- Plasma Membrane MCC/Eisosome Domains Promote Stress Resistance in Fungi.Microbiology and molecular biology reviews : MMBR · 2020Review
- Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole.Biomolecules · 2020Article
- Biochemical Characterization of Yeast Xrn1.Biochemistry · 2020Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- Conformation-dependent partitioning of yeast nutrient transporters into starvation-protective membrane domains.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.FEMS microbiology letters · 2017Article
- MCC/Eisosomes Regulate Cell Wall Synthesis and Stress Responses in Fungi.Journal of fungi (Basel, Switzerland) · 2017Review
- Article
- Article
- Novel mRNA-silencing bodies at the synapse: A never-ending story.Communicative & integrative biologyArticle
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of gene expression on the level of translation and mRNA turnover is widely conserved evolutionarily. We have found that the main mRNA decay enzyme, exoribonuclease Xrn1, accumulates at the plasma membrane-associated eisosomes after glucose exhaustion in a culture of the yeast S. cerevisiae. Eisosomal localization of Xrn1 is not achieved in cells lacking the main component of eisosomes, Pil1, or Sur7, the protein accumulating at the membrane compartment of Can1 (MCC) - the eisosome-organized plasma membrane microdomain. In contrast to the conditions of diauxic shift, when Xrn1 accumulates in processing bodies (P-bodies), or acute heat stress, in which these cytosolic accumulations of Xrn1 associate with eIF3a/Rpg1-containing stress granules, Xrn1 is not accompanied by other mRNA-decay machinery components when it accumulates at eisosomes in post-diauxic cells. It is important that Xrn1 is released from eisosomes after addition of fermentable substrate. We suggest that this spatial segregation of Xrn1 from the rest of the mRNA-decay machinery reflects a general regulatory mechanism, in which the key enzyme is kept separate from the rest of mRNA decay factors in resting cells but ready for immediate use when fermentable nutrients emerge and appropriate metabolism reprogramming is required. In particular, the localization of Xrn1 to the eisosome, together with previously published data, accents the relevance of this plasma membrane-associated compartment as a multipotent regulatory site.
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