ArticlePloS one2015
Multigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. terrestris (Tremellales).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
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- Fungi under Modified Atmosphere-The Effects of COJournal of fungi (Basel, Switzerland) · 2023Article
- Current Fungal Taxonomy and Developments in the Identification System.Current microbiology · 2023Review
- Lung cancer coexisting with Papiliotrema flavescens infection diagnosed by next-generation sequencing: a case report.BMC infectious diseases · 2022Article
- When barcoding fails: Genome chimerization (admixing) and reticulation obscure phylogenetic and taxonomic relationships.Molecular ecology resources · 2022Article
- Fungal Planet description sheets: 1284-1382.Persoonia · 2021Article
- What Is the Best Lens? Comparing the Resolution Power of Genome-Derived Markers and Standard Barcodes.Microorganisms · 2021Article
- Single Strain High-Depth NGS Reveals High rDNA (ITS-LSU) Variability in the Four Prevalent Pathogenic Species of the GenusMicroorganisms · 2021Article
- Effect of Soil Chemical Properties on the Occurrence and Distribution of Entomopathogenic Fungi in Portuguese Grapevine Fields.Pathogens (Basel, Switzerland) · 2021Article
- The evolving species concepts used for yeasts: from phenotypes and genomes to speciation networks.Fungal diversity · 2021Review
- Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi.Microorganisms · 2020Review
- Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species.Studies in mycology · 2020Article
- Selection of yeasts from bee products for alcoholic beverage production.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?IMA fungus · 2020Article
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Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococcus flavescens and C. terrestris are phenotypically indistinguishable sister species that belong to the order Tremellales (Tremellomycetes, Basidiomycota) and which may be mistaken for C. laurentii based on phenotype. Phylogenetic separation between C. flavescens and C. terrestris was based on rDNA sequence analyses, but very little is known on their intraspecific genetic variability or propensity for sexual reproduction. We studied 59 strains from different substrates and geographic locations, and used a multilocus sequencing (MLS) approach complemented with the sequencing of mating type (MAT) genes to assess genetic variation and reexamine the boundaries of the two species, as well as their sexual status. The following five loci were chosen for MLS: the rDNA ITS-LSU region, the rDNA IGS1 spacer, and fragments of the genes encoding the largest subunit of RNA polymerase II (RPB1), the translation elongation factor 1 alpha (TEF1) and the p21-activated protein kinase (STE20). Phylogenetic network analyses confirmed the genetic separation of the two species and revealed two additional cryptic species, for which the names Cryptococcus baii and C. ruineniae are proposed. Further analyses of the data revealed a high degree of genetic heterogeneity within C. flavescens as well as evidence for recombination between lineages detected for this species. Strains of C. terrestris displayed higher levels of similarity in all analysed genes and appear to make up a single recombining group. The two MAT genes (STE3 and SXI1/SXI2) sequenced for C. flavescens strains confirmed the potential for sexual reproduction and suggest the presence of a tetrapolar mating system with a biallelic pheromone/receptor locus and a multiallelic HD locus. In C. terrestris we could only sequence STE3, which revealed a biallelic P/R locus. In spite of the strong evidence for sexual recombination in the two species, attempts at mating compatible strains of both species on culture media were unsuccessful.
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