ArticlePloS one2015
Effect of receptor dimerization on membrane lipid raft structure continuously quantified on single cells by camera based fluorescence correlation spectroscopy.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- The LDL receptor is regulated by membrane cholesterol as revealed by fluorescence fluctuation analysis.Biophysical journal · 2023Article
- Patterning principles of morphogen gradients.Open biology · 2022Review
- Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins.iScience · 2021Article
- Review
- Lipid Rafts: Controversies Resolved, Mysteries Remain.Trends in cell biology · 2020Review
- Caveolae and lipid sorting: Shaping the cellular response to stress.The Journal of cell biology · 2020Review
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- Statistical Analysis of Scanning Fluorescence Correlation Spectroscopy Data Differentiates Free from Hindered Diffusion.ACS nano · 2018Article
- Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy.Nano letters · 2018Article
- Quantifying spatial and temporal variations of the cell membrane ultra-structure by bimFCS.Methods (San Diego, Calif.) · 2018Review
- Complex dynamics at the nanoscale in simple biomembranes.Scientific reports · 2017Article
- Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs.The Journal of cell biology · 2017Article
- Short-Lived Cages Restrict Protein Diffusion in the Plasma Membrane.Scientific reports · 2016Article
- Spot size variation FCS in simulations of the 2D Ising model.Journal of physics D: Applied physics · 2016Article
- Raft-based interactions of gangliosides with a GPI-anchored receptor.Nature chemical biology · 2016Article
- Plasma Membrane Organization of Epidermal Growth Factor Receptor in Resting and Ligand-Bound States.Biophysical journal · 2015Article
- Current approaches to studying membrane organization.F1000Research · 2015Review
- The C99 domain of the amyloid precursor protein resides in the disordered membrane phase.The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Membrane bound cell signaling is modulated by the membrane ultra-structure, which itself may be affected by signaling. However, measuring the interaction of membrane proteins with membrane structures in intact cells in real-time poses considerable challenges. In this paper we present a non-destructive fluorescence method that quantifies these interactions in single cells, and is able to monitor the same cell continuously to observe small changes. This approach combines total internal fluorescence microscopy with fluorescence correlation spectroscopy to measure the protein's diffusion and molecular concentration in different sized areas simultaneously. It correctly differentiates proteins interacting with membrane fences from proteins interacting with cholesterol-stabilized domains, or lipid rafts. This method detects small perturbations of the membrane ultra-structure or of a protein's tendency to dimerize. Through continuous monitoring of single cells, we demonstrate how dimerization of GPI-anchored proteins increases their association with the structural domains. Using a dual-color approach we study the effect of dimerization of one GPI-anchored protein on another type of GPI-anchored protein expressed in the same cell. Scans over the cell surface reveal a correlation between cholesterol stabilized domains and membrane cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.