Evidence map›Paper›PMID 25811194›Full record

ArticlePloS one2015

Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.

Emma Pirilä, Otto Väyrynen, Elias Sundquist, Kaisa Päkkilä, Pia Nyberg, Sini Nurmenniemi, Virve Pääkkönen, Paula Pesonen, Dan Dayan, Marilena Vered and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 3 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 3 syntheses or guidelines pooled it, 47 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Article
  5. Review
  6. Immune cell topography of head and neck cancer.Journal for immunotherapy of cancer · 2024
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  16. The Microenvironment of Tongue Cancer.Advances in experimental medicine and biology · 2020
    Review
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  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Emma PiriläOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Otto VäyrynenOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Elias SundquistOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Kaisa PäkkiläOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Pia NybergOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Sini NurmenniemiOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland.
Virve PääkkönenDepartment of Pedodontics, Cariology and Endodontology, University of Oulu, Oulu, Finland.
Paula PesonenDepartment of Community Dentistry, Institute of Dentistry, University of Oulu, Oulu, Finland.
Dan DayanDepartment of Oral Pathology and Oral Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
Marilena VeredDepartment of Oral Pathology and Oral Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel; Institute of Pathology, The Chaim Sheba Medical Center, Tel Hashomer, Tel Aviv, Israel.
Lars Uhlin-HansenInstitute of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway; Department of Pathology, University Hospital of Northern Norway, Tromsø, Tromsø, Norway.
Tuula SaloOulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland; Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland; Oulu University Hospital, Oulu, Finland.
University of Oulu · FIOulu University Hospital · FITel Aviv University · ILUniversity Hospital of North Norway · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oral tongue squamous cell carcinoma (OTSCC) has a high mortality rate and the incidence is rising worldwide. Despite advances in treatment, the disease lacks specific prognostic markers and treatment modality. The spreading of OTSCC is dependent on the tumor microenvironment and involves tumor-associated macrophages (TAMs). Although the presence of TAMs is associated with poor prognosis in OTSCC, the specific mechanisms underlying this are still unknown. The aim here was to investigate the effect of macrophages (Mfs) on HSC-3 tongue carcinoma cells and NF-kappaB activity. We polarized THP-1 cells to M1 (inflammatory), M2 (TAM-like) and R848 (imidazoquinoline-treated) type Mfs. We then investigated the effect of Mfs on HSC-3 cell migration and NF-kappaB activity, cytokine production and invasion using several different in vitro migration models, a human 3D tissue invasion model, antibody arrays, confocal microscopy, immunohistochemistry and a mouse invasion model. We found that in co-culture studies all types of Mfs fused with HSC-3 cells, a process which was partially due to efferocytosis. HSC-3 cells induced expression of epidermal growth factor and transforming growth factor-beta in co-cultures with M2 Mfs. Direct cell-cell contact between M2 Mfs and HSC-3 cells induced migration and invasion of HSC-3 cells while M1 Mfs reduced HSC-3 cell invasion. M2 Mfs had an excess of NF-kappaB p50 subunit and a lack of p65 subunits both in the presence and absence of HSC-3 cells, indicating dysregulation and pro-tumorigenic NF-kappaB activation. TAM-like cells were abundantly present in close vicinity to carcinoma cells in OTSCC patient samples. We conclude that M2 Mfs/TAMs have an important role in OTSCC regulating adhesion, migration, invasion and cytokine production of carcinoma cells favouring tumor growth. These results demonstrate that OTSCC patients could benefit from therapies targeting TAMs, polarizing TAM-like M2 Mfs to inflammatory macrophages and modulating NF-kappaB activity.

Indexed as

AnimalsBiomarkersCarcinoma, Squamous CellCell CommunicationCell Line, TumorCell MovementCells, CulturedCoculture TechniquesCytokinesDisease Models, AnimalEndocytosisHeterograftsHumansMacrophagesMiceNeoplasm InvasivenessBiomarkersCytokinesNF-kappa B

Identifiers

PMID25811194
PMCPMC4374792
OpenAlexW2032132846

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.