Evidence map›Paper›PMID 25810872›Full record

ArticleIranian journal of basic medical sciences2015

Human Wharton's jelly-derived mesenchymal stem cells express oocyte developmental genes during co-culture with placental cells.

Hamid Reza Asgari, Mohammad Akbari, Mehdi Abbasi, Jafar Ai, Morteza Korouji, Fereshte Aliakbari, Kehinde Adebayo Babatunde, Fereydoon Sargolzaei Aval, Mohammad Taghi Joghataei

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Article in Iranian journal of basic medical sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.

  1. Pooled it
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  8. Review
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  11. Therapeutic Potential of HUMSCs in Female Reproductive Aging.Frontiers in cell and developmental biology · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hamid Reza AsgariDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad AkbariDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mehdi AbbasiDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Jafar AiDepartment of Tissue engineering, School of Advanced Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Morteza KoroujiDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Fereshte AliakbariDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Kehinde Adebayo BabatundeDepartment of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Fereydoon Sargolzaei AvalDepartment of Anatomy, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Mohammad Taghi JoghataeiDepartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Tehran University of Medical Sciences · IRIran University of Medical Sciences · IRZahedan University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. Much more efforts have been made to develop a procedure for attaining germ cells in vitro. Recently, human umbilical cord-derived mesenchymal stem cells (HUMSCs) have been introduced with higher efficacy for differentiation. In this work, we tried to explore the efficacy of HUMSCs and some effective products of placental cells such as transforming growth factors. This study is aimed to optimize a co-culture condition for HUMSCs with placental cells to obtain primordial germ cells (PGCs) and reach into oocyte-like cells in vitro. MATERIALS AND

methodsIn this experimental study, HUMSCs and placental cells were co-cultured for 14 days without any external inducer in vitro. Then HUMSCs were assessed for expression of PGC markers; Octamer-binding transcription factor 4(OCT4), Tyrosine-protein kinase Kit (CKIT), Stage specific embryonic antigen 4 (SSEA4), DEAD (Asp-Glu-Ala-Asp) box polypeptide 4(DDX4) and oocyte specific markers; Growth differentiation factor-9(GDF9), Zona pellucida glycoprotein 3(ZP3). The pertinent markers were assessed by immunocytochemistry and Q-PCR.

resultsCo-cultured HUMSCs with placental cells (including amniotic and chorionic cells) presented Oct4 and DDX4, primordial germ cells specific markers significantly, but increment in expression of oocyte-like cell specific markers, GDF9 and ZP3 did not reach to statistically significant threshold.

conclusionPlacental cell supplements Transforming growth factor (TGF α, β) and basic fibroblast growth factor (bFGF) in a co-culture model can provide proper environment for induction of HUMSCs into PGCs and expression of oocyte-like markers.

Indexed as

Co-cultureHuman umbilical cordMesenchymal stem cellsOocyte-like cellPlacental cells

Identifiers

PMID25810872
PMCPMC4366739
OpenAlexW1842990826

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.