ArticleJournal of neurophysiology2015
Freshly dissociated mature hippocampal astrocytes exhibit passive membrane conductance and low membrane resistance similarly to syncytial coupled astrocytes.
Article in Journal of neurophysiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- The Astroglia Syncytial Theory of Consciousness.International journal of molecular sciences · 2025Review
- Article
- Chronic Stress Impairs the Structure and Function of Astrocyte Networks in an Animal Model of Depression.Neurochemical research · 2023Article
- β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes.Cells · 2022Article
- Microglia maintain the normal structure and function of the hippocampal astrocyte network.Glia · 2022Article
- On the electrical passivity of astrocyte potassium conductance.Journal of neurophysiology · 2021Review
- Approaches to Study Gap Junctional Coupling.Frontiers in cellular neuroscience · 2021Review
- GABAFrontiers in aging neuroscience · 2021Article
- Ion Channels and Electrophysiological Properties of Astrocytes: Implications for Emergent Stimulation Technologies.Frontiers in cellular neuroscience · 2021Review
- Article
- Epileptiform Neuronal Discharges Impair Astrocyte Syncytial Isopotentiality in Acute Hippocampal Slices.Brain sciences · 2020Article
- Astrocyte syncytium: a functional reticular system in the brain.Neural regeneration research · 2019Article
- Ventral midbrain astrocytes display unique physiological features and sensitivity to dopamine D2 receptor signaling.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2019Article
- Syncytial isopotentiality: A system-wide electrical feature of astrocytic networks in the brain.Glia · 2018Article
- Versatile control of synaptic circuits by astrocytes: where, when and how?Nature reviews. Neuroscience · 2018Review
- Dissipation of transmembrane potassium gradient is the main cause of cerebral ischemia-induced depolarization in astrocytes and neurons.Experimental neurology · 2018Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes.Molecular neurobiology · 2016Article
- Electrophysiological behavior of neonatal astrocytes in hippocampal stratum radiatum.Molecular brain · 2016Article
- Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
Mature astrocytes exhibit a linear current-to-voltage K(+) membrane conductance (passive conductance) and an extremely low membrane resistance (Rm) in situ. The combination of these electrophysiological characteristics establishes a highly negative and stable membrane potential that is essential for basic functions, such as K(+) spatial buffering and neurotransmitter uptake. However, astrocytes are coupled extensively in situ. It remains to be determined whether the observed passive behavior and low Rm are attributable to the intrinsic properties of membrane ion channels or to gap junction coupling in functionally mature astrocytes. In the present study, freshly dissociated hippocampal tissues were used as a new model to examine this basic question in young adult animals. The morphologically intact single astrocytes could be reliably dissociated from animals postnatal day 21 and older. At this animal age, dissociated single astrocytes exhibit passive conductance and resting membrane potential similar to those exhibited by astrocytes in situ. To precisely measure the Rm from single astrocytes, dual-patch single-astrocyte recording was performed. We show that dissociated single astrocytes exhibit a low Rm similarly to syncytial coupled astrocytes. Functionally, the symmetric expression of high-K(+) conductance enabled rapid change in the intracellular K(+) concentrations in response to changing K(+) drive force. Altogether, we demonstrate that freshly dissociated tissue preparation is a highly useful model for study of the functional expression and regulation of ion channels, receptors, and transporters in astrocytes and that passive behavior and low Rm are the intrinsic properties of mature astrocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.