ArticlePlant physiology2015
Quantifying protein synthesis and degradation in Arabidopsis by dynamic 13CO2 labeling and analysis of enrichment in individual amino acids in their free pools and in protein.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 135 citations in OpenAlex.
- Systemic and Local Regulation of Root Growth by Vascular Trehalose 6-Phosphate is Correlated With Re-allocation of Primary Metabolites Between Shoots and Roots.Plant, cell & environment · 2026Article
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- Time- and space-resolved transcriptional regulation in Arabidopsis thaliana.Plant biology (Stuttgart, Germany) · 2026Article
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism.Nature plants · 2025Article
- Remodelled ribosomal populations synthesize a specific proteome in proliferating plant tissue during cold.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Accounting for the impact of genotype and environment on variation in leaf respiration of wheat in Mexico and Australia.Journal of experimental botany · 2025Article
- Elevated Temperature Effects on Protein Turnover Dynamics inInternational journal of molecular sciences · 2024Article
- Cycloheximide in the nanomolar range inhibits seed germination ofJournal of pesticide science · 2024Article
- Dynamics of mRNA fate during light stress and recovery: from transcription to stability and translation.The Plant journal : for cell and molecular biology · 2024Article
- Prospects and perspectives: inferring physiological and regulatory targets for CAM from molecular and modelling approaches.Annals of botany · 2023Review
- A phospho-dawn of protein modification anticipates light onset in the picoeukaryote Ostreococcus tauri.Journal of experimental botany · 2023Article
- Article
- In vivo protein kinase activity of SnRK1 fluctuates in Arabidopsis rosettes during light-dark cycles.Plant physiology · 2023Article
- Natural variation of respiration-related traits in plants.Plant physiology · 2023Review
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Enhancing crop yields through improvements in the efficiency of photosynthesis and respiration.The New phytologist · 2023Review
- 13CO2 labeling kinetics in maize reveal impaired efficiency of C4 photosynthesis under low irradiance.Plant physiology · 2022Article
- The circadian clock mutant lhy cca1 elf3 paces starch mobilization to dawn despite severely disrupted circadian clock function.Plant physiology · 2022Article
- Rising rates of starch degradation during daytime and trehalose 6-phosphate optimize carbon availability.Plant physiology · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issuedCORRECTIONS.2015
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein synthesis and degradation represent substantial costs during plant growth. To obtain a quantitative measure of the rate of protein synthesis and degradation, we supplied (13)CO2 to intact Arabidopsis (Arabidopsis thaliana) Columbia-0 plants and analyzed enrichment in free amino acids and in amino acid residues in protein during a 24-h pulse and 4-d chase. While many free amino acids labeled slowly and incompletely, alanine showed a rapid rise in enrichment in the pulse and a decrease in the chase. Enrichment in free alanine was used to correct enrichment in alanine residues in protein and calculate the rate of protein synthesis. The latter was compared with the relative growth rate to estimate the rate of protein degradation. The relative growth rate was estimated from sequential determination of fresh weight, sequential images of rosette area, and labeling of glucose in the cell wall. In an 8-h photoperiod, protein synthesis and cell wall synthesis were 3-fold faster in the day than at night, protein degradation was slow (3%-4% d(-1)), and flux to growth and degradation resulted in a protein half-life of 3.5 d. In the starchless phosphoglucomutase mutant at night, protein synthesis was further decreased and protein degradation increased, while cell wall synthesis was totally inhibited, quantitatively accounting for the inhibition of growth in this mutant. We also investigated the rates of protein synthesis and degradation during leaf development, during growth at high temperature, and compared synthesis rates of Rubisco large and small subunits of in the light and dark.
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