Evidence map›Paper›PMID 25810095›Full record

ArticlePlant physiology2015

The conserved endoribonuclease YbeY is required for chloroplast ribosomal RNA processing in Arabidopsis.

Jinwen Liu, Wenbin Zhou, Guifeng Liu, Chuanping Yang, Yi Sun, Wenjuan Wu, Shenquan Cao, Chong Wang, Guanghui Hai, Zhifeng Wang and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 62 citations in OpenAlex.

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  6. How RNases Shape Mitochondrial Transcriptomes.International journal of molecular sciences · 2022
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  13. Mutation of theFrontiers in plant science · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Jinwen LiuState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Wenbin ZhouState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.) wbzhou@mpimp-golm.mpg.de chengyuxiang@nefu.edu.cn.
Guifeng LiuState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Chuanping YangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Yi SunState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Wenjuan WuState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Shenquan CaoState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Chong WangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Guanghui HaiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Zhifeng WangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Ralph BockState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Jirong HuangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.).
Yuxiang ChengState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China (J.L., G.L., C.Y., S.C., C.W., G.H., Z.W., Y.C.);College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China (J.L.);National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China (W.Z., Y.S., W.W., J.H.); andMax-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany (R.B.) wbzhou@mpimp-golm.mpg.de chengyuxiang@nefu.edu.cn.ORCID http://orcid.org/0000-0002-8530-6687
Institute of Plant Genetics, Polish Academy of Sciences · PLChinese Academy of Sciences · CNHeilongjiang Bayi Agricultural University · CNMax Planck Institute of Molecular Plant Physiology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Maturation of chloroplast ribosomal RNAs (rRNAs) comprises several endoribonucleolytic and exoribonucleolytic processing steps. However, little is known about the specific enzymes involved and the cleavage steps they catalyze. Here, we report the functional characterization of the single Arabidopsis (Arabidopsis thaliana) gene encoding a putative YbeY endoribonuclease. AtYbeY null mutants are seedling lethal, indicating that AtYbeY function is essential for plant growth. Knockdown plants display slow growth and show pale-green leaves. Physiological and ultrastructural analyses of atybeY mutants revealed impaired photosynthesis and defective chloroplast development. Fluorescent microcopy analysis showed that, when fused with the green fluorescence protein, AtYbeY is localized in chloroplasts. Immunoblot and RNA gel-blot assays revealed that the levels of chloroplast-encoded subunits of photosynthetic complexes are reduced in atybeY mutants, but the corresponding transcripts accumulate normally. In addition, atybeY mutants display defective maturation of both the 5' and 3' ends of 16S, 23S, and 4.5S rRNAs as well as decreased accumulation of mature transcripts from the transfer RNA genes contained in the chloroplast rRNA operon. Consequently, mutant plants show a severe deficiency in ribosome biogenesis, which, in turn, results in impaired plastid translational activity. Furthermore, biochemical assays show that recombinant AtYbeY is able to cleave chloroplast rRNAs as well as messenger RNAs and transfer RNAs in vitro. Taken together, our findings indicate that AtYbeY is a chloroplast-localized endoribonuclease that is required for chloroplast rRNA processing and thus for normal growth and development.

Indexed as

Conserved SequenceAmino Acid SequenceArabidopsisArabidopsis ProteinsBase SequenceChloroplastsEndoribonucleasesGene Expression Regulation, PlantGenes, PlantGenome, PlantGreen Fluorescent ProteinsMolecular Sequence DataMutationPhenotypePhotosynthesisPlant LeavesArabidopsis ProteinsEndoribonucleasesGreen Fluorescent ProteinsProtein SubunitsRNA, ChloroplastRNA, MessengerRNA, RibosomalYbeY protein, Arabidopsis

Identifiers

PMID25810095
PMCPMC4424013
OpenAlexW1922497225

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