ArticleJournal of biomedical materials research. Part A2015
Coculture of dorsal root ganglion neurons and differentiated human corneal stromal stem cells on silk-based scaffolds.
Article in Journal of biomedical materials research. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Three-Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Stem Cells and Tissue Engineering-Based Therapeutic Interventions: Promising Strategies to Improve Peripheral Nerve Regeneration.Cellular and molecular neurobiology · 2023Review
- The Chick Embryo and Its Structures as a Model System for Experimental Ophthalmology.Bulletin of experimental biology and medicine · 2023Article
- Article
- Applications of nanomaterials in tissue engineering.RSC advances · 2021Review
- Fabrication and Biocompatibility of Electroconductive Silk Fibroin/PEDOT: PSS Composites for Corneal Epithelial Regeneration.Polymers · 2020Article
- Induction of Irritation and Inflammation in a 3D Innervated Tissue Model of the Human Cornea.ACS biomaterials science & engineering · 2020Article
- Assembly and Application of a Three-Dimensional Human Corneal Tissue Model.Current protocols in toxicology · 2019Article
- Corneal pain and experimental model development.Progress in retinal and eye research · 2019Review
- Current and novel polymeric biomaterials for neural tissue engineering.Journal of biomedical science · 2018Review
- Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves.Scientific reports · 2017Article
- In vitro 3D corneal tissue model with epithelium, stroma, and innervation.Biomaterials · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Corneal tissue displays the highest peripheral nerve density in the human body. Engineering of biomaterials to promote interactions between neurons and corneal tissue could provide tissue models for nerve/cornea development, platforms for drug screening, as well as innovative opportunities to regenerate cornea tissue. The focus of this study was to develop a coculture system for differentiated human corneal stromal stem cells (dhCSSCs) and dorsal root ganglion neurons (DRG) to mimic the human cornea tissue interactions. Axon extension, connectivity, and neuron cell viability were studied. DRG neurons developed longer axons when cocultured with dhCSSCs in comparison to neuron cultures alone. To assess the mechanism involved in the coculture response, nerve growth factors (NGF) secreted by dhCSSCs including NGF, brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), and neurotrophin-3 were characterized with greater focus on BDNF secretion. DhCSSCs also secreted collagen type I, an extracellular matrix molecule favorable for neuronal outgrowth. This coculture system provides a slowly degrading silk matrix to study neuronal responses in concert with hCSSCs related to innervation of corneal tissue with utility toward human corneal nerve regeneration and associated diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.