ArticleGene therapy2015
Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo.
Article in Gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Optogenetics in Pancreatic Islets: Actuators and Effects.Diabetes · 2024Review
- Optogenetic therapeutic strategies for diabetes mellitus.Journal of diabetes · 2024Review
- Optogenetic Approach in Trigeminal Neuralgia and Potential Concerns: Preclinical Insights.Molecular neurobiology · 2024Review
- Light-stimulated insulin secretion from pancreatic islet-like organoids derived from human pluripotent stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Current Topics of Optogenetics for Medical Applications Toward Therapy.Advances in experimental medicine and biology · 2021Article
- Light control of RTK activity: from technology development to translational research.Chemical science · 2020Review
- Optogenetics: Therapeutic spark in neuropathic pain.Bosnian journal of basic medical sciences · 2019Review
- Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- The role of beta cell heterogeneity in islet function and insulin release.Journal of molecular endocrinology · 2018Review
- Optogenetic regulation of insulin secretion in pancreatic β-cells.Scientific reports · 2017Article
- Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics.PloS one · 2017Article
- Shining Light on the Sprout of Life: Optogenetics Applications in Stem Cell Research and Therapy.The Journal of membrane biology · 2016Review
- Engineering of optogenetic devices for biomedical applications in mammalian synthetic biology.Engineering biologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study assessed the ability of optogenetics techniques to provide a better understanding of the control of insulin secretion, particularly regarding pancreatic β-cell function in homeostasis and pathological conditions such as diabetes mellitus (DM). We used optogenetics to investigate whether insulin secretion and blood glucose homeostasis could be controlled by regulating intracellular calcium ion concentrations ([Ca(2+)]i) in a mouse pancreatic β-cell line (MIN6) transfected with the optogenetic protein channelrhodopsin-2 (ChR2). The ChR2-transfected MIN6 (ChR2-MIN6) cells secreted insulin following irradiation with a laser (470 nm). The increase in [Ca(2+)]i was accompanied by elevated levels of messenger RNAs that encode calcium/calmodulin-dependent protein kinase II delta and adenylate cyclase 1. ChR2-MIN6 cells suspended in matrigel were inoculated into streptozotocin-induced diabetic mice that were then subjected to a glucose tolerance test. Laser irradiation of these mice caused a significant decrease in blood glucose, and the irradiated implanted cells expressed insulin. These findings demonstrate the power of optogenetics to precisely and efficiently controlled insulin secretion by pancreatic β-cells 'on demand', in contrast to techniques using growth factors or chemical inducers. Optogenetic technology shows great promise for understanding the mechanisms of glucose homeostasis and for developing treatments for metabolic diseases such as DM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.