Evidence map›Paper›PMID 25809463›Full record

ArticleGene therapy2015

The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.

M Pensak, S Hong, A Dukas, B Tinsley, H Drissi, A Tang, M Cote, O Sugiyama, A Lichtler, D Rowe and 1 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

M PensakDepartment of Orthopaedic Surgery, Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA.
S HongDepartment of Computer Science and Engineering, University of Connecticut, Storrs, CT, USA.
A DukasDepartment of Orthopaedic Surgery, Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA.
B TinsleyDepartment of Orthopaedic Surgery, Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA.
H DrissiDepartment of Orthopaedic Surgery, Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA.
A TangDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
M CoteDepartment of Orthopaedic Surgery, Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA.
O SugiyamaDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
A LichtlerDepartment of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA.
D RoweDepartment of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA.
J R LiebermanDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
UConn Health · USUniversity of Southern California · USUniversity of Connecticut · US

Funding

The Use of Regional Gene Delivery to Heal Critical Sized Bone DefectsR01AR057076 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI LIEBERMAN, JAY R. · 2010 to 2021
$3.9M
NIAMS NIH HHS 7R01AR057076-04
6 · The paper itself

Abstract

The role that transduced mouse bone marrow stromal cells (mBMSCs) engineered to overexpress human bone morphogenetic protein 2 (BMP-2) play in healing critical-sized skeletal defects is largely unknown. We evaluated the interaction between host osteoprogenitor cells and donor mBMSCs transduced with either a lentiviral (LV) vector-expressing red fluorescent protein (RFP) with or without BMP-2 that were implanted into a critical-sized femoral defect. Radiographs taken at the time of killing were evaluated using a five-point scaled scoring system. Frozen histologic sections were analyzed to assess both the transduced cells' role in bone repair and the local osteoprogenitor response. There was complete radiographic bridging in 94% of group I (LV-RFPch-BMP-2-cmyc) and 100% of group III (recombinant human BMP-2) specimens. Radiographs demonstrated a lack of healing in group II (LV-RFPch). Mouse BMSCs transduced with an LV-RFPch-BMP-2 vector were able to induce host cells to differentiate down an osteoblastic lineage and heal a critical-sized defect. However, the donor cells appeared to be functioning as a delivery vehicle of BMP-2 rather than actually differentiating into osteoblasts capable of participating in bone repair as evidenced by a lack of colocalization of the transduced cells to the sites of skeletal repair where the host progenitor cells were found.

Indexed as

Bone RegenerationWound HealingAnimalsBone Marrow CellsBone Morphogenetic Protein 2Cells, CulturedFemurGenetic VectorsHumansMaleMiceMice, TransgenicRecombinant ProteinsStromal CellsTibiaTransduction, GeneticBone Morphogenetic Protein 2recombinant human bone morphogenetic protein-2Recombinant ProteinsTransforming Growth Factor beta

Identifiers

PMID25809463
OpenAlexW2001644736

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.