ArticleThe Plant journal : for cell and molecular biology2015
Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed, 63 citations in OpenAlex.
- Precision RNAi in Tomato Using Synthetic Trans-Acting Small Interfering RNAs Derived From Minimal Precursors.Plant biotechnology journal · 2026Article
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing.The Plant journal : for cell and molecular biology · 2026Article
- Gene silencing in plants by artificial small RNAs derived from minimal precursors and expressed via tobacco rattle virus.Plant molecular biology · 2025Article
- Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors.Bio-protocol · 2025Article
- NNature communications · 2025Article
- A pipeline for validation of Serendipita indica effector-like sRNA suggests cross-kingdom communication in the symbiosis with Arabidopsis.Journal of experimental botany · 2025Article
- Article
- ATG6 interacting with NPR1 increaseseLife · 2025Article
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors.Nucleic acids research · 2025Article
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Principles of miRNA/miRNA* function in plant MIRNA processing.Nucleic acids research · 2024Article
- Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors.Nucleic acids research · 2023Article
- A high-efficiency gene silencing in plants using two-hit asymmetrical artificial MicroRNAs.Plant biotechnology journal · 2023Article
- Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection.Journal of experimental botany · 2023Article
- Targeted suppression of gibberellin biosynthetic genes ZmGA20ox3 and ZmGA20ox5 produces a short stature maize ideotype.Plant biotechnology journal · 2022Article
- Systemic silencing of an endogenous plant gene by two classes of mobile 21-nucleotide artificial small RNAs.The Plant journal : for cell and molecular biology · 2022Article
- MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice.Frontiers in plant science · 2021Review
- Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants.Plant physiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective and large-scale synthesis. Here, a series of expression vectors based on Oryza sativa MIR390 (OsMIR390) precursor was developed for high-throughput cloning and high expression of amiRNAs in monocots. Four different amiRNA sequences designed to target specifically endogenous genes and expressed from OsMIR390-based vectors were validated in transgenic Brachypodium distachyon plants. Surprisingly, amiRNAs accumulated to higher levels and were processed more accurately when expressed from chimeric OsMIR390-based precursors that include distal stem-loop sequences from Arabidopsis thaliana MIR390a (AtMIR390a). In all cases, transgenic plants displayed the predicted phenotypes induced by target gene repression, and accumulated high levels of amiRNAs and low levels of the corresponding target transcripts. Genome-wide transcriptome profiling combined with 5'-RLM-RACE analysis in transgenic plants confirmed that amiRNAs were highly specific.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.