ArticleBiophysical journal2015
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 46 citations in OpenAlex.
- Ultrasensitive Characterization of Native Bacterial Biofilms via Dynamic Nuclear Polarization-Enhanced Solid-State NMR.Angewandte Chemie (International ed. in English) · 2025Article
- Optimizing phage-based mutant recovery and minimizing heat effect in the construction of transposon libraries in Staphylococcus aureus.Scientific reports · 2024Article
- CPMAS NMR platform for direct compositional analysis of mycobacterial cell-wall complexes and whole cells.Journal of magnetic resonance open · 2023Article
- Tapping into the native Pseudomonas bacterial biofilm structure by high-resolution multidimensional solid-state NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2023Article
- Detection of intact vancomycin-arginine as the active antibacterial conjugate inRSC medicinal chemistry · 2023Article
- Solid-state NMR molecular snapshots ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.Chemical reviews · 2022Review
- Antimicrobial Peptide Mechanisms Studied by Whole-Cell Deuterium NMR.International journal of molecular sciences · 2022Review
- Chemical Biology Tools for Examining the Bacterial Cell Wall.Cell chemical biology · 2020Review
- Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.Biochemical Society transactions · 2020Review
- Wall teichoic acids govern cationic gold nanoparticle interaction with Gram-positive bacterial cell walls.Chemical science · 2020Article
- Spectral comparisons of mammalian cells and intact organelles by solid-state NMR.Journal of structural biology · 2019Article
- A Dual-Function Antibiotic-Transporter Conjugate Exhibits Superior Activity in Sterilizing MRSA Biofilms and Killing Persister Cells.Journal of the American Chemical Society · 2018Article
- Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance.Biochemistry · 2018Article
- Whole Ribosome NMR: Dipolar Couplings and Contributions to Whole Cells.The journal of physical chemistry. B · 2017Article
- Specific binding of a naturally occurring amyloidogenic fragment of Streptococcus mutans adhesin P1 to intact P1 on the cell surface characterized by solid state NMR spectroscopy.Journal of biomolecular NMR · 2016Article
- In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.Biophysical journal · 2015Review
- Frequency-selective REDOR and spin-diffusion relays in uniformly labeled whole cells.Solid state nuclear magnetic resonance · 2015Article
- Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- A new understanding of antibiotic action via solid-state NMR of cells with uniform isotopic labeling.Biophysical journal · 2015Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
Gram-positive bacteria surround themselves with a thick cell wall that is essential to cell survival and is a major target of antibiotics. Quantifying alterations in cell-wall composition are crucial to evaluating drug modes of action, particularly important for human pathogens that are now resistant to multiple antibiotics such as Staphylococcus aureus. Macromolecular and whole-cell NMR spectroscopy allowed us to observe the full panel of carbon and nitrogen pools in S. aureus cell walls and intact whole cells. We discovered that one-dimensional (13)C and (15)N NMR spectra, together with spectroscopic selections based on dipolar couplings as well as two-dimensional spin-diffusion measurements, revealed the dramatic compositional differences between intact cells and cell walls and allowed the identification of cell-wall signatures in whole-cell samples. Furthermore, the whole-cell NMR approach exhibited the sensitivity to detect distinct compositional changes due to treatment with the antibiotics fosfomycin (a cell-wall biosynthesis inhibitor) and chloramphenicol (a protein synthesis inhibitor). Whole cells treated with fosfomycin exhibited decreased peptidoglycan contributions while those treated with chloramphenicol contained a higher percentage of peptidoglycan as cytoplasmic protein content was reduced. Thus, general antibiotic modes of action can be identified by profiling the total carbon pools in intact whole cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.