ArticleBiophysical journal2015
Mechanosensitivity of a rapid bioluminescence reporter system assessed by atomic force microscopy.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Light-based 3D printing of mechanoluminescent living gels loaded with dinoflagellates.Science advances · 2026Article
- Chemical stimulation sustains bioluminescence of living light materials.Science advances · 2026Article
- Bioluminescence of the heterotrophic dinoflagellate Polykrikos kofoidii Chatton 1914 (Dinophyceae).Journal of phycology · 2026Article
- Light-regulated chloroplast morphodynamics in a single-celled dinoflagellate.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Ultrasensitive and robust mechanoluminescent living composites.Science advances · 2023Article
- Bioluminescent Dinoflagellates as a Bioassay for Toxicity Assessment.International journal of molecular sciences · 2022Review
- Highly robust and soft biohybrid mechanoluminescence for optical signaling and illumination.Nature communications · 2022Article
- New Perspectives Related to the Bioluminescent System in Dinoflagellates:International journal of molecular sciences · 2020Review
- Identification of a vacuolar proton channel that triggers the bioluminescent flash in dinoflagellates.PloS one · 2017Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells are sophisticated integrators of mechanical stimuli that lead to physiological, biochemical, and genetic responses. The bioluminescence of dinoflagellates, alveolate protists that use light emission for predator defense, serves as a rapid noninvasive whole-cell reporter of mechanosensitivity. In this study, we used atomic force microscopy (AFM) to explore the relationship between cell mechanical properties and mechanosensitivity in live cells of the dinoflagellate Pyrocystis lunula. Cell stiffness was 0.56 MPa, consistent with cells possessing a cell wall. Cell response depended on both the magnitude and velocity of the applied force. At the maximum stimulation velocity of 390 μm s(-1), the threshold response occurred at a force of 7.2 μN, resulting in a contact time of 6.1 ms and indentation of 2.1 μm. Cells did not respond to a low stimulation velocity of 20 μm s(-1), indicating a velocity dependent response that, based on stress relaxation experiments, was explained by the cell viscoelastic properties. This study demonstrates the use of AFM to study mechanosensitivity in a cell system that responds at fast timescales, and provides insights into how viscoelastic properties affect mechanosensitivity. It also provides a comparison with previous studies using hydrodynamic stimulation, showing the discrepancy in cell response between direct compressive forces using AFM and those within flow fields based on average flow properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.