ArticleMicrobial biotechnology2015
Four-stage dissolved oxygen strategy based on multi-scale analysis for improving spinosad yield by Saccharopolyspora spinosa ATCC49460.
Article in Microbial biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- Optimization of spinosad production by Saccharopolysosa spinosa YJY-12 and its application in controlling Eggplant Thrips.Scientific reports · 2025Article
- Addition of vegetable oil to enhance the biosynthesis of butenyl-spinosyn in a high production strain Saccharopolyspora pogona.PloS one · 2025Article
- A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides.Molecules (Basel, Switzerland) · 2022Article
- Increasing the heterologous production of spinosad inSynthetic and systems biotechnology · 2021Article
- Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production.Scientific reports · 2021Article
- Effect of dissolved oxygen on L-methionine production from glycerol by Escherichia coli W3110BL using metabolic flux analysis method.Journal of industrial microbiology & biotechnology · 2020Article
- Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels.Journal of industrial microbiology & biotechnology · 2017Article
- Dissolved oxygen control strategy for improvement of TL1-1 production in submerged fermentation byBioresources and bioprocessing · 2017Article
- High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea.Applied and environmental microbiology · 2016Article
- A New Medium for Improving Spinosad Production by Saccharopolyspora spinosa.Jundishapur journal of microbiology · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dissolved oxygen (DO) is an important influencing factor in the process of aerobic microbial fermentation. Spinosad is an aerobic microbial-derived secondary metabolite. In our study, spinosad was used as an example to establish a DO strategy by multi-scale analysis, which included a reactor, cell and gene scales. We changed DO conditions that are related to the characteristics of cell metabolism (glucose consumption rate, biomass accumulation and spinosad production). Consequently, cell growth was promoted by maintaining DO at 40% in the first 24 h and subsequently increasing DO to 50% in 24 h to 96 h. In an in-depth analysis of the key enzyme genes (gtt, spn A, spn K and spn O), expression of spinosad and specific Adenosine Triphosphate (ATP), the spinosad yield was increased by regulating DO to 30% within 96 h to 192 h and then changing it to 25% in 192 h to 240 h. Under the four-phase DO strategy, spinosad yield increased by 652.1%, 326.1%, 546.8%, and 781.4% compared with the yield obtained under constant DO control at 50%, 40%, 30%, and 20% respectively. The proposed method provides a novel way to develop a precise DO strategy for fermentation.
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