ArticleOpen biology2015
A novel method for the purification of inositol phosphates from biological samples reveals that no phytate is present in human plasma or urine.
Article in Open biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed, 160 citations in OpenAlex.
- A Hybrid Experimental and in silico Platform for ITPK1 Chemical Probe Discovery.SLAS discovery : advancing life sciences R & D · 2026Article
- Analytical methods and tools for studying inositol phosphates.FEBS letters · 2026Review
- Micronutrients in Future Diets: Considerations for Dietary Iron and the Food Matrix Effects on Bioavailability.Molecular nutrition & food research · 2026Review
- Climate change and the antinutrient-antioxidant puzzle in common bean seeds.Journal of the science of food and agriculture · 2026Review
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- Phosphorus-specific, liquid chromatography inductively coupled plasma mass spectrometry for analysis of inositol phosphate and inositol pyrophosphate metabolism.The Biochemical journal · 2025Article
- A linear pathway for inositol pyrophosphate metabolism revealed by 18O labeling and model reduction.PLoS computational biology · 2025Article
- Article
- Conservation of heat stress acclimation by the IPK2-type kinases that control the synthesis of the inositol pyrophosphate 4/6-InsP7 in land plants.PLoS genetics · 2025Article
- Phytic acid impairs macrophage inflammatory response in Echinococcus multilocularis infection.Communications biology · 2025Article
- Pools of Independently Cycling Inositol Phosphates Revealed by Pulse Labeling withJournal of the American Chemical Society · 2025Article
- Basic features of cellular inositol metabolism as revealed by a newly developed LC-MS method.The Biochemical journal · 2025Article
- Synthesis of a New Purine Analogue Class with Antifungal Activity and Improved Potency against Fungal IPACS infectious diseases · 2025Article
- Inositol phosphates as an overlooked phosphorous source in marine ecosystems.The ISME journal · 2025Article
- Cell Extraction and Enrichment of Inositol Polyphosphate and Inositol Pyrophosphates Using Titanium Dioxide.Methods in molecular biology (Clifton, N.J.) · 2025Article
- IP6 Kinase Inhibitor Screening Assays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Analysis of Inositol Phosphates, Diphosphoinositol Phosphates, and Their Analogs by HPLC on an Acid-Compatible Stationary Phase with Detection by Complexation with Ferric Ion.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Capillary Electrophoresis Mass Spectrometry for Inositol (Pyro)phosphate Profiling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Determination of Steady-State Inositol Phosphate Profile of the Liverwort, Marchantia polymorpha, Using SAX-HPLC.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Using HILIC-MS/MS Method to Study Inositol Pyrophosphate.Methods in molecular biology (Clifton, N.J.) · 2025Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Inositol phosphates are a large and diverse family of signalling molecules. While genetic studies have discovered important functions for them, the biochemistry behind these roles is often not fully characterized. A key obstacle in inositol phosphate research in mammalian cells has been the lack of straightforward techniques for their purification and analysis. Here we describe the ability of titanium dioxide (TiO2) beads to bind inositol phosphates. This discovery allowed the development of a new purification protocol that, coupled with gel analysis, permitted easy identification and quantification of InsP6 (phytate), its pyrophosphate derivatives InsP7 and InsP8, and the nucleotides ATP and GTP from cell or tissue extracts. Using this approach, InsP6, InsP7 and InsP8 were visualized in Dictyostelium extracts and a variety of mammalian cell lines and tissues, and the effects of metabolic perturbation on these were explored. TiO2 bead purification also enabled us to quantify InsP6 in human plasma and urine, which led to two distinct but related observations. Firstly, there is an active InsP6 phosphatase in human plasma, and secondly, InsP6 is undetectable in either fluid. These observations seriously question reports that InsP6 is present in human biofluids and the advisability of using InsP6 as a dietary supplement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.