ArticleThe Journal of cell biology1985
Monoclonal antibody to intermediate filament antigen cross-reacts with higher plant cells.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 74 citations in OpenAlex.
- Cell cycle-dependent dynamics of a plant intermediate filament motif protein with intracellular localization related to microtubules.Protoplasma · 2020Article
- Evidence for nuclear interaction of a cytoskeleton protein (OsIFL) with metallothionein and its role in salinity stress tolerance.Scientific reports · 2016Article
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- Article
- The Arabidopsis cytoskeletal genome.The arabidopsis book · 2003Article
- Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales).Folia microbiologica · 2002Article
- Tubulin Isotypes in Rye Roots Are Altered during Cold Acclimation.Plant physiology · 1990Article
- Reconstitution of intermediate filaments from a higher plant.The Biochemical journal · 1989Article
- Microtubule-binding proteins from carrot : I. Initial characterization and microtubule bundling.Planta · 1989Article
- An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus.The Journal of cell biology · 1987Article
- The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.The EMBO journal · 1987Article
- The dynamic state of heat shock proteins in chicken embryo fibroblasts.The Journal of cell biology · 1986Article
- Distribution of calmodulin in pea seedlings: Immunocytochemical localization in plumules and root apices.Planta · 1986Article
- The molecular evolution of actin.Genetics · 1986Article
- Intermediate filaments in muscle and epithelial cells of nematodes.The Journal of cell biology · 1986Article
- The distribution of cytoplasmic microtubules throughout the cell cycle of the centric diatom Stephanopyxis turris: their role in nuclear migration and positioning the mitotic spindle during cytokinesis.The Journal of cell biology · 1986Article
- Identification of multiple tubulins in taxol microtubules purified from carrot suspension cells.The EMBO journal · 1985Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monoclonal antibody (anti-IFA) raised (Pruss et al., 1981, Cell 27:419-428) against an intermediate filament antigen, which is widespread throughout phylogeny, has been shown here to cross-react with higher plants. On immunoblotting, anti-IFA cross-reacted with proteins in homogenates of carrot suspension cells and of meristematic cells from onion root tips. A 50-kD cross-reactive protein was enriched in a fraction that consisted of detergent-insoluble bundles of 7-nm fibrils from carrot protoplasts (Powell et al., 1982, J. Cell Sci. 56:319-335). By use of indirect immunofluorescence, anti-IFA stained formaldehyde-fixed onion meristematic cells and carrot protoplasts in patterns approximating those obtained with monoclonal anti-tubulins. That anti-IFA was not recognizing plant tubulins was established by use of immunoblots of two-dimensional gels on which the proteins that comprised isolated fibrillar bundles and taxol-purified carrot tubulins had been separated. The two groups of proteins had different positional coordinates: anti-IFA recognized the fibrillar bundle proteins, and anti-tubulins recognized plant microtubule proteins with no cross-reaction to the heterologous proteins. Likewise, formaldehyde-fixed taxol microtubules from carrot cells could be stained with anti-tubulin but not with anti-IFA. It is concluded that an epitope common to intermediate filaments from animals co-distributes with microtubules in higher plant cells.
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