ArticlePloS one2015
Improving transformation of Staphylococcus aureus belonging to the CC1, CC5 and CC8 clonal complexes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Genetic modification of intractable bacterial clones by heat shock-facilitated phage transduction.Cell reports methods · 2026Article
- Exploring human-to-food contamination potential: Genome-based analysis of diversity, toxin repertoire, and antimicrobial resistance of methicillin-resistantOne health (Amsterdam, Netherlands) · 2025Article
- CRISPR-Cas9 enables efficient genome engineering of the strictly lytic, broad-host-range staphylococcal bacteriophage K.Applied and environmental microbiology · 2025Article
- Characterization of theFrontiers in microbiology · 2023Article
- Mechanism of DNA cleavage by the endonuclease SauUSI: a major barrier to horizontal gene transfer and antibiotic resistance in Staphylococcus aureus.Nucleic acids research · 2021Article
- Single-nucleotide polymorphisms in a vancomycin-resistant Staphylococcus aureus strain based on whole-genome sequencing.Archives of microbiology · 2020Article
- Roles of lytic transglycosylases in biofilm formation and β-lactam resistance in methicillin-resistantAntimicrobial agents and chemotherapy · 2019Article
- CspA regulation of Staphylococcus aureus carotenoid levels and σMolecular microbiology · 2019Article
- Systematic evasion of the restriction-modification barrier in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Highly efficient base editing inChemical science · 2018Article
- Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference.PloS one · 2018Article
- Bypassing the Restriction System To Improve Transformation of Staphylococcus epidermidis.Journal of bacteriology · 2017Article
- DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen found in hospital and community environments that can cause serious infections. A major barrier to genetic manipulations of clinical isolates has been the considerable difficulty in transforming these strains with foreign plasmids, such as those from E. coli, in part due to the type I and IV Restriction Modification (R-M) barriers. Here we combine a Plasmid Artificial Modification (PAM) system with DC10B E. coli cells (dcm mutants) to bypass the barriers of both type I and IV R-M of S. aureus, thus allowing E. coli plasmid DNA to be transformed directly into clinical MRSA strains MW2, N315 and LAC, representing three of the most common clonal complexes. Successful transformation of clinical S. aureus isolates with E. coli-derived plasmids should greatly increase the ability to genetically modify relevant S. aureus strains and advance our understanding of S. aureus pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.