ArticlePloS one2015
Force dependent biotinylation of myosin IIA by α-catenin tagged with a promiscuous biotin ligase.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5.International journal of molecular sciences · 2022Article
- Unique and redundant functions of cytoplasmic actins and nonmuscle myosin II isoforms at epithelial junctions.Annals of the New York Academy of Sciences · 2022Review
- Spatial proximity of proteins surrounding zyxin under force-bearing conditions.Molecular biology of the cell · 2021Article
- Strategies to Investigate Ubiquitination in Huntington's Disease.Frontiers in chemistry · 2020Review
- The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics.Journal of cell science · 2019Article
- A simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat.Biochemical and biophysical research communications · 2017Article
- Biochemical analysis of force-sensitive responses using a large-scale cell stretch device.Cell adhesion & migration · 2017Article
- E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Filling the Void: Proximity-Based Labeling of Proteins in Living Cells.Trends in cell biology · 2016Review
- Meet the neighbors: Mapping local protein interactomes by proximity-dependent labeling with BioID.Proteomics · 2016Review
- An improved smaller biotin ligase for BioID proximity labeling.Molecular biology of the cell · 2016Article
- BioID Identification of Lamin-Associated Proteins.Methods in enzymology · 2016Article
- Recent advances in large-scale protein interactome mapping.F1000Research · 2016Review
- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Tissues and organs undergo constant physical perturbations and individual cells must respond to mechanical forces to maintain tissue integrity. However, molecular interactions underlying mechano-transduction are not fully defined at cell-cell junctions. This is in part due to weak and transient interactions that are likely prevalent in force-induced protein complexes. Using in situ proximal biotinylation by the promiscuous biotin ligase BirA tagged to α-catenin and a substrate stretch cell chamber, we sought to identify force-dependent molecular interactions surrounding α-catenin, an actin regulator at the sites of cadherin mediated cell-cell adhesion. While E-cadherin, β-catenin, vinculin and actin localize with α-catenin at cell-cell contacts in immuno-fluorescent staining, only β-catenin and plakoglobin were biotinylated, suggesting that this proximal biotinylation is limited to the molecules that are in the immediate vicinity of α-catenin. In mechanically stretched samples, increased biotinylation of non-muscle myosin IIA, but not myosin IIB, suggests close spatial proximity between α-catenin and myosin IIA during substrate stretching. This force-induced biotinylation diminished as myosin II activity was inhibited by blebbistatin. Taken together, this promising technique enables us to identify force sensitive complexes that may be essential for mechano-responses in force bearing cell adhesion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.