ArticlePloS one2015
Abnormal pregnancy outcomes in mice using an induced periodontitis model and the haematogenous migration of Fusobacterium nucleatum sub-species to the murine placenta.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Periodontitis during pregnancy: The effect on the gut microbiome and intestinal inflammation.Journal of periodontology · 2026Article
- The rise and evolving role ofCurrent research in microbial sciences · 2025Review
- Advances in the relationship between periodontopathogens and respiratory diseases (Review).Molecular medicine reports · 2024Review
- Characterisation of Mid-Gestation Amniotic Fluid Cytokine and Bacterial DNA Profiles in Relation to Pregnancy Outcome in a Small Australian Cohort.Microorganisms · 2023Article
- Article
- Gut and Reproductive Tract Microbiota Adaptation during Pregnancy: New Insights for Pregnancy-Related Complications and Therapy.Microorganisms · 2021Review
- Role of Maternal Infections and Inflammatory Responses on Craniofacial Development.Frontiers in oral health · 2021Review
- Low AbundanceFrontiers in immunology · 2021Article
- Article
- A survey ofMicrobial genomics · 2020Article
- Evidence that intra-amniotic infections are often the result of an ascending invasion - a molecular microbiological study.Journal of perinatal medicine · 2019Article
- Structural insight into the high reduction potentials observed for Fusobacterium nucleatum flavodoxin.Protein science : a publication of the Protein Society · 2019Article
- Periodontal Disease and Pregnancy Outcomes: Overview of Systematic Reviews.JDR clinical and translational research · 2018Review
- Role of Maternal Periodontitis in Preterm Birth.Frontiers in immunology · 2017Review
- Clinical significance ofOncoTargets and therapy · 2017Article
- Reexamining the role ofGut microbesReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate if there is subspecies specific migration to the placenta by Fusobacterium nucleatum (Fn) and to determine whether experimentally induced periodontitis results in adverse pregnancy outcomes (APO) in mice.
methodsPeriodontitis was induced in pregnant mice using an inoculum of Fn and Porphyromonas gingivalis. In parallel, four sub-species of Fn were individually injected into the circulatory system. At day 18 of gestation, the placenta, liver, spleen and blood were harvested and litter size, number of viable fetuses and resorptions, maternal, fetal and placenta weights were recorded. For the direct inoculation group, some mice were allowed to deliver for assessment of length of gestation, litter size, maternal, placental and pup weight. The presence of Fn was assessed by PCR and inflammatory mediators were measured by ELISA or multiplex analysis.
resultsMice with alveolar bone loss, a marker of periodontitis, demonstrated significantly higher fetal weights (p = 0.015) and fetal/placental weight ratios (p = 0.030). PCR analysis of maternal organs did not identify Fn in any extracted tissues. In mice that received direct injection of Fn subspecies, varying degrees of APO were observed including preterm birth, intrauterine growth restriction, and fetal loss. Haematogenous spread of only Fn subsp. nucleatum to the placenta was confirmed. Litter size was significantly smaller (p = 0.023) and the number of resorptions was higher in inoculated versus control groups. Mice injected with subsp. nucleatum had significantly increased circulating CRP levels (p = 0.020) compared to controls while the mice with induced periodontitis had increased levels of IL-6 (p = 0.047) and IL-8 (p = 0.105).
conclusionsPeriodontitis in mice elevated fetal weight and the fetal weight/placental weight ratio. This study found that subsp. nucleatum migrated haematogenously to the placenta, leading to APO in mice. The study supports the potential role of Fn in the association between periodontitis and APO.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.