ArticleFrontiers in plant science2015
Citrus PH5-like H(+)-ATPase genes: identification and transcript analysis to investigate their possible relationship with citrate accumulation in fruits.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 54 citations in OpenAlex.
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- ClaPEPCK4: target gene for breeding innovative watermelon germplasm with low malic acid and high sweetness.GM crops & food · 2025Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.International journal of molecular sciences · 2025Article
- Distinctive acidity in citrus fruit is linked to loss of proanthocyanidin biosynthesis.iScience · 2024Article
- Article
- Transcriptome Analysis and VIGS Identification of Key Genes Regulating Citric Acid Metabolism in Citrus.Current issues in molecular biology · 2023Article
- Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression.BMC plant biology · 2022Article
- The Interaction Between CitMYB52 and CitbHLH2 Negatively Regulates Citrate Accumulation by ActivatingFrontiers in plant science · 2022Article
- MYB308-mediated transcriptional activation of plasma membrane HHorticulture research · 2022Article
- Transcriptome analysis of the pulp of citrus fruitlets suggests that domestication enhanced growth processes and reduced chemical defenses increasing palatability.Frontiers in plant science · 2022Article
- Metabolomic and transcriptomic analyses reveal the mechanism of sweet-acidic taste formation during pineapple fruit development.Frontiers in plant science · 2022Article
- High-density linkage map construction and identification of loci regulating fruit quality traits in blueberry.Horticulture research · 2021Article
- High-spatiotemporal-resolution transcriptomes provide insights into fruit development and ripening in Citrus sinensis.Plant biotechnology journal · 2021Article
- Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles.Horticulture research · 2020Article
- Citrus Taste Modification Potentials by Genetic Engineering.International journal of molecular sciences · 2019Review
- A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple.Plant biotechnology journal · 2019Article
- Hyperacidification of Citrus fruits by a vacuolar proton-pumping P-ATPase complex.Nature communications · 2019Article
- Primary Metabolism in Citrus Fruit as Affected by Its Unique Structure.Frontiers in plant science · 2019Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PH5 is a petunia gene that encodes a plasma membrane H(+)-ATPase and determines the vacuolar pH. The citrate content of fruit cell vacuoles influences citrus organoleptic qualities. Although citrus could have PH5-like homologs that are involved in citrate accumulation, the details are still unknown. In this study, extensive data-mining with the PH5 sequence and PCR amplification confirmed that there are at least eight PH5-like genes (CsPH1-8) in the citrus genome. CsPHs have a molecular mass of approximately 100 kDa, and they have high similarity to PhPH5, AtAHA10 or AtAHA2 (from 64.6 to 80.9%). They contain 13-21 exons and 12-20 introns and were evenly distributed into four subgroups of the P3A-subfamily (CsPH1, CsPH2, and CsPH3 in Group I, CsPH4 and CsPH5 in Group II, CsPH6 in Group IV, and CsPH7 and CsPH8 in Group III together with PhPH5). A transcript analysis showed that CsPH1, 3, and 4 were predominantly expressed in mature leaves, whereas CsPH2 and 7 were predominantly expressed in roots, CsPH5 and 6 were predominantly expressed in flowers, and CsPH8 was predominantly expressed in fruit juice sacs (JS). Moreover, the CsPH transcript profiles differed between orange and pummelo, as well as between high-acid and low-acid cultivars. The low-acid orange "Honganliu" exhibits low transcript levels of CsPH3, CsPH4, CsPH5, and CsPH8, whereas the acid-free pummelo (AFP) has only a low transcript level of CsPH8. In addition, ABA injection increased the citrate content significantly, which was accompanied by the obvious induction of CsPH2, 6, 7, and 8 transcript levels. Taken together, we suggest that CsPH8 seems likely to regulate citrate accumulation in the citrus fruit vacuole.
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