ArticleIndian journal of microbiology2015
Insights into the Origin of Clostridium botulinum Strains: Evolution of Distinct Restriction Endonuclease Sites in rrs (16S rRNA gene).
Article in Indian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 20 citations in OpenAlex.
- Complete genomic sequence and analysis of β2 toxin gene mapping of Clostridium perfringens JXJA17 isolated from piglets in China.Scientific reports · 2021Article
- Comparison of DNA Extraction Methods for Optimal Recovery of Metagenomic DNA from Human and Environmental Samples.Indian journal of microbiology · 2019Article
- Aligning Microbial Biodiversity for Valorization of Biowastes: Conception to Perception.Indian journal of microbiology · 2019Review
- Methylation content sensitive enzyme ddRAD (MCSeEd): a reference-free, whole genome profiling system to address cytosine/adenine methylation changes.Scientific reports · 2019Article
- An In Silico Approach for Identification of the Pathogenic Species, Helicobacter pylori and Its Relatives.Indian journal of microbiology · 2016Article
- Identification of DNA Methyltransferase Genes in Human Pathogenic Bacteria by Comparative Genomics.Indian journal of microbiology · 2016Article
- Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification.Indian journal of microbiology · 2016Article
- Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria.Indian journal of microbiology · 2016Review
- A Genome-Wide Profiling Strategy as an Aid for Searching Unique Identification Biomarkers for Streptococcus.Indian journal of microbiology · 2016Article
- Genome Wide Search for Biomarkers to Diagnose Yersinia Infections.Indian journal of microbiology · 2015Article
- Genome Wide Analysis for Rapid Identification of Vibrio Species.Indian journal of microbiology · 2015Article
- Genome Wide Analysis for Searching Novel Markers to Rapidly Identify Clostridium Strains.Indian journal of microbiology · 2015Article
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5 authors at 2 institutions in 1 country.
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Abstract
Diversity analysis of Clostridium botulinum strains is complicated by high microheterogeneity caused by the presence of 9-22 copies of rrs (16S rRNA gene). The need is to mine genetic markers to identify very closely related strains. Multiple alignments of the nucleotide sequences of the 212 rrs of 13 C. botulinum strains revealed intra- and inter-genomic heterogeneity. Low intragenomic heterogeneity in rrs was evident in strains 230613, Alaska E43, Okra, Eklund 17B, Langeland, 657, Kyoto, BKT015925, and Loch Maree. The most heterogenous rrs sequences were those of C. botulinum strains ATCC 19397, Hall, H04402065, and ATCC 3502. In silico restriction mapping of these rrs sequences was observable with 137 type II Restriction endonucleases (REs). Nucleotide changes (NC) at these RE sites resulted in appearance of distinct and additional sites, and loss in certain others. De novo appearances of RE sites due to NC were recorded at different positions in rrs gene. A nucleotide transition A>G in rrs of C. botulinum Loch Maree and 657 resulted in the generation of 4 and 10 distinct RE sites, respectively. Transitions A>G, G>A, and T>C led to the loss of RE sites. A perusal of the entire NC and in silico RE mapping of rrs of C. botulinum strains provided insights into their evolution. Segregation of strains on the basis of RE digestion patterns of rrs was validated by the cladistic analysis involving six house keeping genes: dnaN, gyrB, metG, prfA, pyrG, and Rho.
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