ReviewJournal of biomolecular screening2015
Flow Cytometry: Impact on Early Drug Discovery.
Review in Journal of biomolecular screening, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Unveiling and stratifying cell cycle-dependent drug efficacy using a single-cell PLOM-CON approach with correlation anomaly and presage protein signals.Communications biology · 2025Article
- Essential Fluidics for a Flow Cytometer.Current protocols · 2024Article
- Article
- The Transporter-Mediated Cellular Uptake and Efflux of Pharmaceutical Drugs and Biotechnology Products: How and Why Phospholipid Bilayer Transport Is Negligible in Real Biomembranes.Molecules (Basel, Switzerland) · 2021Review
- Inhibition of the HEG1-KRIT1 interaction increases KLF4 and KLF2 expression in endothelial cells.FASEB bioAdvances · 2021Article
- Creating and screening natural product libraries.Natural product reports · 2020Review
- Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells.Analytical chemistry · 2019Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Sizing biological cells using a microfluidic acoustic flow cytometer.Scientific reports · 2019Article
- A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery.SLAS discovery : advancing life sciences R & D · 2018Article
- High-Throughput Flow Cytometry Drug Combination Discovery with Novel Synergy Analysis Software, SynScreen.SLAS discovery : advancing life sciences R & D · 2018Article
- High-Throughput Screening Approach for Identifying Compounds That Inhibit Nonhomologous End Joining.SLAS discovery : advancing life sciences R & D · 2018Article
- Impact of a five-dimensional framework on R&D productivity at AstraZeneca.Nature reviews. Drug discovery · 2018Article
- Line-Focused Optical Excitation of Parallel Acoustic Focused Sample Streams for High Volumetric and Analytical Rate Flow Cytometry.Analytical chemistry · 2017Article
- Multiplexed Lipid Bilayers on Silica Microspheres for Analytical Screening Applications.Analytical chemistry · 2017Article
- Biologically Relevant Heterogeneity: Metrics and Practical Insights.SLAS discovery : advancing life sciences R & D · 2017Review
- Targeting efflux pumps to overcome antifungal drug resistance.Future medicinal chemistry · 2016Review
- Quantitative Magnetic Separation of Particles and Cells Using Gradient Magnetic Ratcheting.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Cell-Based Assay Design for High-Content Screening of Drug Candidates.Journal of microbiology and biotechnology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Modern flow cytometers can make optical measurements of 10 or more parameters per cell at tens of thousands of cells per second and more than five orders of magnitude dynamic range. Although flow cytometry is used in most drug discovery stages, "sip-and-spit" sampling technology has restricted it to low-sample-throughput applications. The advent of HyperCyt sampling technology has recently made possible primary screening applications in which tens of thousands of compounds are analyzed per day. Target-multiplexing methodologies in combination with extended multiparameter analyses enable profiling of lead candidates early in the discovery process, when the greatest numbers of candidates are available for evaluation. The ability to sample small volumes with negligible waste reduces reagent costs, compound usage, and consumption of cells. Improved compound library formatting strategies can further extend primary screening opportunities when samples are scarce. Dozens of targets have been screened in 384- and 1536-well assay formats, predominantly in academic screening lab settings. In concert with commercial platform evolution and trending drug discovery strategies, HyperCyt-based systems are now finding their way into mainstream screening labs. Recent advances in flow-based imaging, mass spectrometry, and parallel sample processing promise dramatically expanded single-cell profiling capabilities to bolster systems-level approaches to drug discovery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.