ArticleDevelopment (Cambridge, England)2015
Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Directed and efficient generation of calretinin interneurons from human pluripotent stem cells.Stem cell research & therapy · 2025Article
- Gestational iron deficiency affects the ratio between interneuron subtypes in the postnatal cerebral cortex in mice.Development (Cambridge, England) · 2023Article
- Cortical interneuron development is affected in 4H leukodystrophy.Brain : a journal of neurology · 2023Article
- Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome.Yonago acta medica · 2023Article
- Article
- Transcriptional heterogeneity of ventricular zone cells in the ganglionic eminences of the mouse forebrain.eLife · 2022Article
- The organization and development of cortical interneuron presynaptic circuits are area specific.Cell reports · 2021Article
- Development, Diversity, and Death of MGE-Derived Cortical Interneurons.International journal of molecular sciences · 2021Review
- Two-Phase Lineage Specification of Telencephalon Progenitors Generated From Mouse Embryonic Stem Cells.Frontiers in cell and developmental biology · 2021Article
- Direct reprogramming into interneurons: potential for brain repair.Cellular and molecular life sciences : CMLS · 2019Review
- Article
- Studying and modulating schizophrenia-associated dysfunctions of oligodendrocytes with patient-specific cell systems.NPJ schizophrenia · 2018Review
- Embryonic stem cell transplants as a therapeutic strategy in a rodent model of autism.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Multiple roles of Sonic Hedgehog in the developing human cortex are suggested by its widespread distribution.Brain structure & function · 2018Article
- Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors.Journal of visualized experiments : JoVE · 2017Article
- Animal Models of Developmental Neuropathology in Schizophrenia.Schizophrenia bulletin · 2017Article
- Stem cell-derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model.Molecular psychiatry · 2017Article
- Hippocampal GABAergic Inhibitory Interneurons.Physiological reviews · 2017Review
- Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration.Cell stem cell · 2017Article
- Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells.Stem cell reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
Medial ganglionic eminence (MGE)-derived GABAergic cortical interneurons (cINs) consist of multiple subtypes that are involved in many cortical functions. They also have a remarkable capacity to migrate, survive and integrate into cortical circuitry after transplantation into postnatal cortex. These features have engendered considerable interest in generating distinct subgroups of interneurons from pluripotent stem cells (PSCs) for the study of interneuron fate and function, and for the development of cell-based therapies. Although advances have been made, the capacity to generate highly enriched pools of subgroup fate-committed interneuron progenitors from PSCs has remained elusive. Previous studies have suggested that the two main MGE-derived interneuron subgroups--those expressing somatostatin (SST) and those expressing parvalbumin (PV)--are specified in the MGE from Nkx2.1-expressing progenitors at higher or lower levels of sonic hedgehog (Shh) signaling, respectively. To further explore the role of Shh and other factors in cIN fate determination, we generated a reporter line such that Nkx2.1-expressing progenitors express mCherry and postmitotic Lhx6-expressing MGE-derived interneurons express GFP. Manipulations of Shh exposure and time in culture influenced the subgroup fates of ESC-derived interneurons. Exposure to higher Shh levels, and collecting GFP-expressing precursors at 12 days in culture, resulted in the strongest enrichment for SST interneurons over those expressing PV, whereas the strongest enrichment for PV interneurons was produced by lower Shh and by collecting mCherry-expressing cells after 17 days in culture. These findings confirm that fate determination of cIN subgroups is crucially influenced by Shh signaling, and provide a system for the further study of interneuron fate and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.