ArticlePloS one2015
Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Review
- Multidrug resistance and biofilm formation among bacterial isolates from chronic wounds in animals: evaluation of anti-biofilm activity.Frontiers in veterinary science · 2026Article
- Quantification of Enzymatic Biofilm Removal Using the Sauerbrey Equation: Application to the Case ofACS omega · 2024Article
- Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment.Microorganisms · 2023Review
- Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni.Journal of applied genetics · 2022Article
- Biofilms: Formation, drug resistance and alternatives to conventional approaches.AIMS microbiology · 2022Review
- Review
- AMicrobial genomics · 2021Article
- Antimicrobial Resistance Gene Transfer from Campylobacter jejuni in Mono- and Dual-Species Biofilms.Applied and environmental microbiology · 2021Article
- Molecular Mechanisms of Campylobacter Biofilm Formation and Quorum Sensing.Current topics in microbiology and immunology · 2021Review
- Bridging the Gap: A Role forMicroorganisms · 2020Review
- Retail liver juices enhance the survivability of Campylobacter jejuni and Campylobacter coli at low temperatures.Scientific reports · 2019Article
- Article
- Article
- Biofilm Formation in Different Salmonella Serotypes Isolated from Poultry.Current microbiology · 2019Article
- Extracellular DNA in natural environments: features, relevance and applications.Applied microbiology and biotechnology · 2018Review
- Antibiofilm Effect of DNase against Single and Mixed Species Biofilm.Foods (Basel, Switzerland) · 2018Article
- Environmental Stress-Induced Bacterial Lysis and Extracellular DNA Release Contribute to Campylobacter jejuni Biofilm Formation.Applied and environmental microbiology · 2018Article
- Intrinsic and Extrinsic Aspects onFrontiers in microbiology · 2017Article
- Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA inFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The fastidious nature of the foodborne bacterial pathogen Campylobacter jejuni contrasts with its ability to survive in the food chain. The formation of biofilms, or the integration into existing biofilms by C. jejuni, is thought to contribute to food chain survival. As extracellular DNA (eDNA) has previously been proposed to play a role in C. jejuni biofilms, we have investigated the role of extracellular DNases (eDNases) produced by C. jejuni in biofilm formation. A search of 2791 C. jejuni genomes highlighted that almost half of C. jejuni genomes contains at least one eDNase gene, but only a minority of isolates contains two or three of these eDNase genes, such as C. jejuni strain RM1221 which contains the cje0256, cje0566 and cje1441 eDNase genes. Strain RM1221 did not form biofilms, whereas the eDNase-negative strains NCTC 11168 and 81116 did. Incubation of pre-formed biofilms of NCTC 11168 with live C. jejuni RM1221 or with spent medium from a RM1221 culture resulted in removal of the biofilm. Inactivation of the cje1441 eDNase gene in strain RM1221 restored biofilm formation, and made the mutant unable to degrade biofilms of strain NCTC 11168. Finally, C. jejuni strain RM1221 was able to degrade genomic DNA from C. jejuni NCTC 11168, 81116 and RM1221, whereas strain NCTC 11168 and the RM1221 cje1441 mutant were unable to do so. This was mirrored by an absence of eDNA in overnight cultures of C. jejuni RM1221. This suggests that the activity of eDNases in C. jejuni affects biofilm formation and is not conducive to a biofilm lifestyle. These eDNases do however have a potential role in controlling biofilm formation by C. jejuni strains in food chain relevant environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.