ArticlePloS one2015
Detection and characterisation of Lactobacillus spp. in the bovine uterus and their influence on bovine endometrial epithelial cells in vitro.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Lacticaseibacillus rhamnosus GR-1 Alleviates Escherichia coli-induced Autophagy-dependent Ferroptosis in Bovine Endometrial Epithelial Cells via FOXO1 Signaling.Probiotics and antimicrobial proteins · 2026Article
- Bacterial and Fungal Dynamics of the Uterine Microbiota in Postpartum Beef Cows Supplemented with Grape Pomace.Animals : an open access journal from MDPI · 2026Article
- Insights into population adaptation and biodiversity of lactic acid bacteria in challenged date palm leaves silaging, using MALDI-TOF MS.Current research in microbial sciences · 2024Article
- Endometrial responses to bacterial and viral infection: a scoping review.Human reproduction update · 2023Article
- Dynamics and Diversity of Intrauterine Anaerobic Microbiota in Dairy Cows with Clinical and Subclinical Endometritis.Animals : an open access journal from MDPI · 2022Article
- Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle.Microorganisms · 2022Article
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- Article
- Beneficial Effects of Lactic Acid Bacteria on Animal Reproduction Function.Veterinary medicine international · 2022Review
- Association of intrauterine presence of Lactobacillus spp. with inflammation and pathogenic bacteria in the uterus in postpartum dairy cows.The Journal of reproduction and development · 2021Article
- Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitis.Journal of animal science and biotechnology · 2021Article
- Honokiol inhibits endoplasmic reticulum stress-associated lipopolysaccharide-induced inflammation and apoptosis in bovine endometrial epithelial cells.Experimental and therapeutic medicine · 2021Article
- Safety, environmental and technological characterization of beneficial autochthonous lactic bacteria, and their vaginal administration to pregnant cows for the design of homologous multi-strain probiotic formulas.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Development of Bacterial Therapeutics against the Bovine Respiratory Pathogen Mannheimia haemolytica.Applied and environmental microbiology · 2019Article
- Influence of intrauterine administration of Lactobacillus buchneri on reproductive performance and pro-inflammatory endometrial mRNA expression of cows with subclinical endometritis.Scientific reports · 2018Article
- Uterine Microbiota of Dairy Cows With Clinical and Subclinical Endometritis.Frontiers in microbiology · 2018Article
- Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality.PloS one · 2018Article
- Bovine Endometrial Epithelial Cells Scale Their Pro-inflammatory ResponseFrontiers in cellular and infection microbiology · 2017Article
- Article
- mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages.Reproductive biology and endocrinology : RB&E · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial infections and inflammation of the uterus are common in dairy cattle after parturition. In particular, pathogenic bacteria that cause endometritis have been the focus of research in cattle reproduction in the last ten years. The aim of the present study was to identify commensal lactobacilli in the bovine uterus and to examine their influence on the synthesis of pro-inflammatory factors in bovine endometrial epithelial cells in vitro. Lactobacillus species were isolated from healthy bovine uteri and further characterised. Bovine endometrial epithelial cells in the second passage (n = 5 animals) were co-cultured with the autochthonous isolates L. buchneri, L. ruminis and L. amylovorus as well as with a commercially available L. vaginalis in different multiplicities of infection (MOI = 1, 5 and 10, respectively). Endometrial epithelial cells cultured without bacteria served as controls. At distinct points in time (2, 4 and 6 h) total RNA was extracted from co-cultured epithelial cells and subjected to reverse transcription quantitative PCR of pro-inflammatory factors. Furthermore, the release of such factors by co-cultured epithelial cells was measured by ELISA or EIA after 24 and 48 h. L. ruminis and L. amylovorus induced increased interleukin (IL) IL1A, IL6, IL8 and prostaglandin-endoperoxide synthase 2 mRNA levels and the release of IL8 and prostaglandin F(2α) in endometrial epithelial cells compared with control cells. In contrast, L. buchneri did not significantly influence the expression and release of these factors. Toll-like receptors 2 and 6 transcripts were found unchanged in co-cultured and untreated epithelial cells in vitro. However, endometrial epithelial cells of each animal showed individual differences in the response to bacterial load. These results suggest that Lactobacillus species are present in the bovine uterus, revealing immunomodulatory properties.
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