ArticleBioMed research international2015
Diagnosis of Neisseria gonorrhoeae using molecular beacon.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- A Triplex PCR Assay for Simultaneous Detection of Non-viral STDs in Both Dry and Wet Swabs, Offering a Rapid and Cost-Effective Diagnostic Tool.The Yale journal of biology and medicine · 2025Article
- Alkhumra Hemorrhagic Fever Virus (AHFV): A Concise Overview.The Yale journal of biology and medicine · 2024Review
- Performance verification and clinical evaluation of the NAP-Fluo Cycler system for detecting five genital tract pathogens based on microfluidic technology.Practical laboratory medicine · 2024Article
- Development of diagnostic tests for pathogen identification and detection of antimicrobial resistance on WHO global priority pathogens using modular real-time nucleic acid amplification test.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Improved management can be achieved by introducing additional parameters in the syndromic diagnosis of nonviral sexually transmitted infections at low-resource settings.AJOG global reports · 2022Article
- Multiple sexually transmitted co-infections are associated with adverse reproductive outcomes in asymptomatic adolescent pregnant women; A Prospective cohort study.Frontiers in medicine · 2022Article
- Evaluating the utility of syndromic case management for three sexually transmitted infections in women visiting hospitals in Delhi, India.Scientific reports · 2017Article
- Illuminating meningococcal diagnosis with LAMP.The Lancet. Infectious diseases · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neisseria gonorrhoeae is an important sexually transmitted diseases (STD) causing pathogen worldwide. Due to absence of an affordable diagnostic assay, routine screening of gonococcal infection becomes impossible in developing countries where infection rates are maximum. Treatment is given on the basis of symptoms alone which leads to spread of infection. Thus, development of a rapid, sensitive, specific, and PCR based visual diagnostic assay suitable for developing countries, required for better disease management, is aimed at in present study. Endocervical swabs were collected from patients visiting gynecology department of various hospitals in Delhi. In-house PCR based assay was developed and modified to visual assay using molecular beacon for end-point detection. It was evaluated against Roche AMPLICOR NG kit and rmp gene. Specificity of beacon was confirmed by competition experiments. Diagnostic test was 98.21% specific and 99.59% sensitive whereas negative and positive predicted value were 99.40% and 98.78%, respectively. We also observed that twice the concentration (2X) of premix was stable at 4°C for 4 months and dry swab samples gave concordant results with that of wet swabs. These features make the test best suitable for routine diagnosis of genital infections in developing countries.
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