ArticleIranian journal of microbiology2014
Survey on O157:H7 enterohemorrhagic Escherichia coli (EHEC) in cattle in Golestan province, Iran.
Article in Iranian journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed.
- Shiga Toxin-ProducingMicroorganisms · 2025Review
- Molecular serotyping of Shiga toxin-producingVeterinary research forum : an international quarterly journal · 2023Article
- Virulence factors, serogroups, and antibiotic resistance of Shiga-toxin producingIranian journal of veterinary research · 2021Article
- Zoonotic implications of camel diseases in Iran.Veterinary medicine and science · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesA diverse group of Escherichia coli are known as enterohemorrhagic Escherichia coli (EHEC) including O157:H7 and non-O157 EHEC. Enterohemorrhagic strains are related to sever clinical conditions in humans including hemorrhagic colitis and hemolytic uremic syndrome, and most of the recorded outbreaks occurred due to O157: H7 E. coli. The aim of the present study was to investigate the presence of O157:H7 E. coli among healthy cattle in Golestan province. MATERIALS AND
methodsFecal samples were collected from 180 clinically healthy cattle in Golestan province. After primary enrichment, samples were streaked on sorbitol MacConkey agar supplemented with cefixime and potassium tellurite (CT-SMAC). Non-sorbitol fermenting (NSF) Escherichia coli isolates were subjected to serotyping using commercial O157 antisera and rfb O157 gene PCR. Isolates were additionally tested for major virulence factors of EHEC including stx1, stx2, eae and ehly by multiplex-PCR.
resultsEighteen NSF isolates were recovered from CT-SMAC confirmed as E. coli in biochemical tests. None of the obtained isolates belonged to O157 serogroup. Overall, two isolates harbored the tested virulence genes; one isolate possessed stx2 and ehly, and the other one carried stx2, eae and ehly.
conclusionThe results of this study indicated that cattle in Golestan province could be the reservoir for non-O157 EHEC.
Indexed as
Identifiers
25802712PMC4367945What OpenQuestion holds
Registered trials
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