Evidence map›Paper›PMID 25802708›Full record

ArticleIranian journal of microbiology2014

Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene.

Hossein Khoshkharam-Roodmajani, Jamal Sarvari, Abdollah Bazargani, Mohammad Reza Kandekar-Ghahraman, Ali Nazari-Alam, Mohammad Motamedifar

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Article in Iranian journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Molecular typing ofVeterinary world · 2018
    Article
  4. Genetic diversity ofIranian journal of microbiology · 2018
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hossein Khoshkharam-RoodmajaniDepartment of Bacteriology and Virology, Faculty of Medicine ; Student Research Committee.
Jamal SarvariDepartment of Bacteriology and Virology, Faculty of Medicine.
Abdollah BazarganiDepartment of Bacteriology and Virology, Faculty of Medicine.
Mohammad Reza Kandekar-GhahramanDepartment of Bacteriology and Virology, Faculty of Medicine.
Ali Nazari-AlamDepartment of Bacteriology and Virology, Faculty of Medicine.
Mohammad MotamedifarDepartment of Bacteriology and Virology, Faculty of Medicine ; Shiraz HIV/AIDS Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesTo investigate coagulase gene polymorphism of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. MATERIALS AND

methodsA total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP.

resultsThere were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme.

conclusionPCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.

Indexed as

Antibiotic resistanceCoagulase geneMRSAPCRRFLP

Identifiers

PMID25802708
PMCPMC4367941

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