ArticlePloS one2015
Enteric neurospheres are not specific to neural crest cultures: implications for neural stem cell therapies.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Altered enteric neurodevelopment in the Ncx knockout mouse model of intestinal neuronal dysplasia.Pediatric surgery international · 2025Article
- A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes.Journal of neuroscience methods · 2024Article
- Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia.Stem cell research & therapy · 2023Article
- Dickkopf1 induces enteric neurogenesis and gliogenesis in vitro if apoptosis is evaded.Communications biology · 2023Article
- Article
- Intestinal Models for Personalized Medicine: from Conventional Models to Microfluidic Primary Intestine-on-a-chip.Stem cell reviews and reports · 2022Review
- Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine.Stem cells translational medicine · 2021Article
- Neural crest-like stem cells for tissue regeneration.Stem cells translational medicine · 2021Review
- Influence of bacterial components on the developmental programming of enteric neurons.Physiological reports · 2020Article
- Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children.Cells · 2019Article
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
- DRG-Derived Neural Progenitors Differentiate into Functional Enteric Neurons Following Transplantation in the Postnatal Colon.Cell transplantation · 2019Article
- Transplanted enteric neural stem cells integrate within the developing chick spinal cord: implications for spinal cord repair.Journal of anatomy · 2018Article
- Recent developments in cell-based ENS regeneration - a short review.Innovative surgical sciences · 2018Review
- Tissue engineering for the treatment of short bowel syndrome in children.Pediatric research · 2018Review
- Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine.Stem cell reports · 2017Article
- Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon.Nature communications · 2017Article
- Exposure to GDNF Enhances the Ability of Enteric Neural Progenitors to Generate an Enteric Nervous System.Stem cell reports · 2017Article
- In vivo transplantation of fetal human gut-derived enteric neural crest cells.Neurogastroenterology and motility · 2017Article
- Optimizing neurogenic potential of enteric neurospheres for treatment of neurointestinal diseases.The Journal of surgical research · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
objectivesEnteric neural stem cells provide hope of curative treatment for enteric neuropathies. Current protocols for their harvesting from humans focus on the generation of 'neurospheres' from cultures of dissociated gut tissue. The study aims to better understand the derivation, generation and composition of enteric neurospheres.
designGut tissue was obtained from Wnt1-Cre;Rosa26Yfp/Yfp transgenic mice (constitutively labeled neural crest cells) and paediatric patients. Gut cells were cultured either unsorted (mixed neural crest/non-neural crest), or following FACS selection into neural crest (murine-YFP+ve/human-p75+ve) or non-neural crest (YFP-ve/p75-ve) populations. Cultures and resultant neurospheres were characterized using immunolabelling in vitro and following transplantation in vivo.
resultsCultures of (i) unsorted, (ii) neural crest, and (iii) non-neural crest cell populations generated neurospheres similar in numbers, size and morphology. Unsorted neurospheres were highly heterogeneous for neural crest content. Neural crest-derived (YFP+ve/p75+ve) neurospheres contained only neural derivatives (neurons and glia) and were devoid of non-neural cells (i.e. negative for SMA, c-Kit), with the converse true for non-neural crest-derived (YFP-ve/p75-ve) 'neurospheres'. Under differentiation conditions only YFP+ve cells gave rise to neural derivatives. Both YFP+ve and YFP-ve cells displayed proliferation and spread upon transplantation in vivo, but YFP-ve cells did not locate or integrate within the host ENS.
conclusionsSpherical accumulations of cells, so-called 'neurospheres' forming in cultures of dissociated gut contain variable proportions of neural crest-derived cells. If they are to be used for ENS cell replacement therapy then improved protocols for their generation, including cell selection, should be sought in order to avoid inadvertent transplantation of non-therapeutic, non-ENS cells.
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