ArticleHistochemistry1985
Automatic determination of labeling density in protein A-gold immunocytochemical preparations using an image analyzer. Application to peroxisomal enzymes.
Article in Histochemistry, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 11 citations in OpenAlex.
- Contributions of the immunogold technique to investigation of the biology of peroxisomes.Histochemistry and cell biology · 1996Review
- Morphometric cytochemistry of diminution of catalase-containing peroxisomes in copper-loaded liver.The Histochemical journal · 1989Article
- Immunogold localization of peroxisomal enzymes in Epon-embedded liver tissue. Enhancement of sensitivity by etching with ethanolic sodium hydroxide.Histochemistry · 1988Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of an automatic image analyzer (TAS, Leitz, Wetzlar) for determination of labeling density in protein A-gold labeled sections is described. Electron micrographs of rat liver labeled with 12 nm gold particles for peroxisomal enzymes are placed on the macrounit of TAS and the images of peroxisomes on TAS-monitor are contoured with a light pen. The instrument measures the surface of the contoured areas. Based on their gray level, the gold particles over the peroxisome are detected automatically and counted and the labeling density for each peroxisome is calculated.
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Registered trials
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