ArticlePloS one2015
Nitric oxide enhances keratinocyte cell migration by regulating Rho GTPase via cGMP-PKG signalling.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Nitric oxide: an old drug but with new horizons in ophthalmology-a narrative review.Annals of translational medicine · 2023Review
- Preparation of therapy-grade extracellular vesicles from adipose tissue to promote diabetic wound healing.Frontiers in bioengineering and biotechnology · 2023Article
- Multifaceted roles of mitochondria in wound healing and chronic wound pathogenesis.Frontiers in cell and developmental biology · 2023Review
- Schlafen4Frontiers in immunology · 2023Article
- Regulatory Role of Nitric Oxide in Cutaneous Inflammation.Inflammation · 2022Review
- Role of nitric oxide in regulating epidermal permeability barrier function.Experimental dermatology · 2022Review
- Signaling pathways in cutaneous wound healing.Frontiers in physiology · 2022Review
- Proteomics changes after negative pressure wound therapy in diabetic foot ulcers.Molecular medicine reports · 2021Article
- A computational model of mutual antagonism in the mechano-signaling network of RhoA and nitric oxide.BMC molecular and cell biology · 2021Article
- Intracellular CaInternational journal of molecular sciences · 2020Article
- Long-term Western diet intake leads to dysregulated bile acid signaling and dermatitis with Th2 and Th17 pathway features in mice.Journal of dermatological science · 2019Article
- The 12-HHT/BLT2/NO Axis Is Associated to the Wound Healing and Skin Condition in Different Glycaemic States.Medical sciences (Basel, Switzerland) · 2019Article
- TLR2 Promotes Monocyte/Macrophage Recruitment Into the Liver and Microabscess Formation to Limit the Spread ofFrontiers in immunology · 2019Article
- Photobiomodulation induces in vitro re-epithelialization via nitric oxide production.Lasers in medical science · 2018Article
- Reversal of stress fibre formation by Nitric Oxide mediated RhoA inhibition leads to reduction in the height of preformed thrombi.Scientific reports · 2018Article
- Article
- ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NFCell death discovery · 2017Article
- Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway.PloS one · 2017Article
- Conditioned Medium from Adipose-Derived Stem Cells (ADSCs) Promotes Epithelial-to-Mesenchymal-Like Transition (EMT-Like) in Glioma Cells In vitro.Molecular neurobiology · 2016Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveNitric oxide (NO) has been shown to improve wound healing, but the mechanism underlying this function is not well defined. Here, we explored the effect of NO on the migration of a human keratinocyte cell line (HaCaT) and its possible mechanism.
methodsThe effects of NO on HaCaT cells in the presence of different concentrations of the NO donor sodium nitroprusside (SNP) were evaluated in a cell migration assay. Subsequently, the cytoskeleton reorganization of cultured HaCaT cells stained with rhodamine-phalloidin was observed with a confocal laser scanning microscope. The mRNA expression and active proteins of CDC42, Rac1 and RhoA in the cultured cells were determined via RT-PCR and pull-down assays, respectively. Furthermore, the roles of various inhibitors or agonists specific to cGMP, PKG and CDC42, Rac1, RhoA in the effects of NO on HaCaT cell migration, F-actin stress fibre formation, and Rho GTPase expression were observed.
resultsIt was also found HaCaT cell migration was increased by SNP in a dose-dependent manner, and the other two NO donors either spermine NONOate or SNAP had almost the same effects on HaCat cell migrations. The formation of F-actin stress fibres in SNP-treated HaCaT cells was increased. The mRNA expression and the active proteins of CDC42, Rac1 and RhoA were found to be upregulated after SNP treatment. Similar effects were observed after the cells were treated with a cGMP or PKG agonist. Additionally, the SNP-mediated upregulation of the mRNA expression and the active proteins of CDC42, Rac1 and RhoA were inhibited by the addition of an inhibitor of cGMP or PKG. Moreover, the SNP-mediated promoting effects of migration and cytoskeleton reorganization were inhibited by treatment with inhibitors of cGMP, PKG, CDC42, Rac1 and RhoA respectively.
conclusionOur data indicated that the stimulatory effects of NO on cell migration of HaCaT cells are mediated by the cGMP signalling pathway via the upregulation of Rho-GTPase expression, which might promote cytoskeleton reorganization.
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