ArticlePLoS pathogens2015
An 18 kDa scaffold protein is critical for Staphylococcus epidermidis biofilm formation.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- The secreted staphylococcal biofilm protein Sbp forms biomolecular condensates in the presence of DNA.Protein science : a publication of the Protein Society · 2026Article
- The Many Lives of a Single Sequence: Functional Plasticity Through Amyloid Polymorphism.Sub-cellular biochemistry · 2026Review
- Staphylococcus epidermidis alters macrophage polarization and phagocytic uptake by extracellular DNA release in vitro.NPJ biofilms and microbiomes · 2024Article
- Host factors abolish the need for polysaccharides and extracellular matrix-binding protein inJournal of medical microbiology · 2021Article
- Virulence Factors in Coagulase-Negative Staphylococci.Pathogens (Basel, Switzerland) · 2021Review
- FightingFrontiers in cellular and infection microbiology · 2021Review
- Biological Functions of Prokaryotic Amyloids in Interspecies Interactions: Facts and Assumptions.International journal of molecular sciences · 2020Review
- The biofilm adhesion protein Aap fromThe Journal of biological chemistry · 2020Article
- Surface Proteins ofFrontiers in microbiology · 2020Review
- Role of Phenol-Soluble Modulins in Staphylococcus epidermidis Biofilm Formation and Infection of Indwelling Medical Devices.Journal of molecular biology · 2019Article
- Redundant and Distinct Roles of Secreted Protein Eap and Cell Wall-Anchored Protein SasG in Biofilm Formation and Pathogenicity of Staphylococcus aureus.Infection and immunity · 2019Article
- Article
- The metalloprotease SepA governs processing of accumulation-associated protein and shapes intercellular adhesive surface properties in Staphylococcus epidermidis.Molecular microbiology · 2017Article
- Identification of Genes Controlled by the Essential YycFG Two-Component System Reveals a Role for Biofilm Modulation inFrontiers in microbiology · 2017Article
- Prevalence of Virulence-Related Determinants in Clinical Isolates ofJundishapur journal of microbiology · 2016Article
- The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response.Microbiology spectrum · 2016Review
- Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming surface protein SasG.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Biofilm Matrix Composition Affects the Susceptibility of Food Associated Staphylococci to Cleaning and Disinfection Agents.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virulence of the nosocomial pathogen Staphylococcus epidermidis is crucially linked to formation of adherent biofilms on artificial surfaces. Biofilm assembly is significantly fostered by production of a bacteria derived extracellular matrix. However, the matrix composition, spatial organization, and relevance of specific molecular interactions for integration of bacterial cells into the multilayered biofilm community are not fully understood. Here we report on the function of novel 18 kDa Small basic protein (Sbp) that was isolated from S. epidermidis biofilm matrix preparations by an affinity chromatographic approach. Sbp accumulates within the biofilm matrix, being preferentially deposited at the biofilm-substratum interface. Analysis of Sbp-negative S. epidermidis mutants demonstrated the importance of Sbp for sustained colonization of abiotic surfaces, but also epithelial cells. In addition, Sbp promotes assembly of S. epidermidis cell aggregates and establishment of multilayered biofilms by influencing polysaccharide intercellular-adhesin (PIA) and accumulation associated protein (Aap) mediated intercellular aggregation. While inactivation of Sbp indirectly resulted in reduced PIA-synthesis and biofilm formation, Sbp serves as an essential ligand during Aap domain-B mediated biofilm accumulation. Our data support the conclusion that Sbp serves as an S. epidermidis biofilm scaffold protein that significantly contributes to key steps of surface colonization. Sbp-negative S. epidermidis mutants showed no attenuated virulence in a mouse catheter infection model. Nevertheless, the high prevalence of sbp in commensal and invasive S. epidermidis populations suggests that Sbp plays a significant role as a co-factor during both multi-factorial commensal colonization and infection of artificial surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.