Evidence map›Paper›PMID 25798926›Full record

ArticlePloS one2015

CCL21/CCR7 enhances the proliferation, migration, and invasion of human bladder cancer T24 cells.

Miao Mo, Mi Zhou, Lu Wang, Lin Qi, Kehua Zhou, Long-Fei Liu, Zhi Chen, Xiong-Bing Zu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
2.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 61 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Miao MoDepartment of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Mi ZhouDepartment of Urology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
Lu WangHealth Management Center, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Lin QiDepartment of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Kehua ZhouDepartment of Health Care Studies, Daemen College, 4380 Main Street, Amherst, NY 14226, United States of America.
Long-Fei LiuDepartment of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Zhi ChenDepartment of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Xiong-Bing ZuDepartment of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
Central South University · CNXiangya Hospital Central South University · CNDaemen College · USZhejiang Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effects of CCL21/CCR7 on the proliferation, migration, and invasion of T24 cells and the possible associated mechanisms: expression of MMP-2 and MMP-9, and regulation of BCL-2 and BAX proteins.

methodsT24 cells received corresponding treatments including vehicle control, antibody (20 ng/mL CCR7 antibody and 50 ng/ml CCL21), and 50, 100, and 200 ng/ml CCL21. Proliferation was evaluated by MTT assay; cell migration and invasion were assayed using a transwell chamber. Cell apoptosis was induced by Adriamycin (ADM). The rate of cell apoptosis was examined by flow cytometry using annexin V-FITC/PI staining. Western-blot was used to analyze MMP-2 and MMP-9 and BCL-2 and BAX proteins.

resultsCCL21 promoted T24 cell proliferation in concentration-dependent manner with that 200 ng/mL induced the largest amount of proliferation. Significant differences of cell migration were found between CCL21treatment groups and the control group in both the migration and invasion studies (P < 0.001 for all). The expressions of MMP-2 and MMP-9 proteins were significantly increased after CCL21 treatment (p < 0.05 for all). Protein expression of Bcl-21 follows an ascending trend while the expression of Bax follows a descending trend as the concentration of CCL21 increases. No difference was found between the control group and antibody group for all assessments.

conclusionCCL21/CCR7 promoted T24 cell proliferation and enhanced its migration and invasion via the increased expression of MMP-2 and MMP-9. CCL21/CCR7 had antiapoptotic activities on T24 cells via regulation of Bcl-2 and Bax proteins. CCL21/CCR7 may promote bladder cancer development and metastasis.

Indexed as

Cell MovementCell ProliferationApoptosisBlotting, WesternCell AdhesionChemokine CCL21Flow CytometryHumansNeoplasm InvasivenessReceptors, CCR7Tumor Cells, CulturedUrinary Bladder NeoplasmsCCL21 protein, humanChemokine CCL21Receptors, CCR7

Identifiers

PMID25798926
PMCPMC4370593
OpenAlexW2141794526

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.