ArticlePloS one2015
Identification of reference genes for real-time quantitative PCR experiments in the liverwort Marchantia polymorpha.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Evolutionarily conserved but not indispensable for survival: plastic UV RESISTANCE LOCUS8 functionality in Marchantia polymorpha under laboratory and field conditions.Journal of experimental botany · 2026Article
- A key role for S-nitrosylation in immune regulation and development in the liverwort Marchantia polymorpha.Journal of experimental botany · 2026Article
- Multiomics analyses of mutants for Marchantia polymorpha FERONIA and MARIS reveal a link between cell wall integrity and abscisic acid responses.Plant & cell physiology · 2026Article
- Conservation and divergence of UVR8 photoreceptor-mediated UV-B signaling in Marchantia polymorpha.Plant physiology · 2026Article
- Involvement of a NIMA-related kinase in cell division of the liverwort Marchantia polymorpha.Plant & cell physiology · 2025Article
- A conserved ARF-DNA interface underlies auxin-triggered transcriptional response.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Simple and Scalable Chopped-Thallus Transformation Method forPlants (Basel, Switzerland) · 2025Article
- Evolutionary origins and functional diversification of Auxin Response Factors.Nature communications · 2024Article
- MurA-catalyzed synthesis of 5-enolpyruvylshikimate-3-phosphate confers glyphosate tolerance in bryophytes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.Development (Cambridge, England) · 2024Article
- Thermospermine Is an Evolutionarily Ancestral Phytohormone Required for Organ Development and Stress Responses in Marchantia Polymorpha.Plant & cell physiology · 2024Article
- MiRNAs differentially expressed in vegetative and reproductive organs ofRNA biology · 2024Article
- Article
- Plant lineage-specific PIKMIN1 drives APC/CCCS52A2 E3-ligase activity-dependent cell division.Plant physiology · 2023Article
- The wound-activated ERF15 transcription factor drivesScience advances · 2022Article
- PIF-independent regulation of growth by an evening complex in the liverwort Marchantia polymorpha.PloS one · 2022Article
- Normal oil body formation inFrontiers in plant science · 2022Article
- Functional analysis of the BEige and Chediak-Higashi domain gene MpFrontiers in plant science · 2022Article
- Article
- The liverwort oil body is formed by redirection of the secretory pathway.Nature communications · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time quantitative polymerase chain reaction (qPCR) has become widely used as a method to compare gene transcript levels across different conditions. However, selection of suitable reference genes to normalize qPCR data is required for accurate transcript level analysis. Recently, Marchantia polymorpha has been adopted as a model for the study of liverwort development and land plant evolution. Identification of appropriate reference genes has therefore become a necessity for gene expression studies. In this study, transcript levels of eleven candidate reference genes have been analyzed across a range of biological contexts that encompass abiotic stress, hormone treatment and different developmental stages. The consistency of transcript levels was assessed using both geNorm and NormFinder algorithms, and a consensus ranking of the different candidate genes was then obtained. MpAPT and MpACT showed relatively constant transcript levels across all conditions tested whereas the transcript levels of other candidate genes were clearly influenced by experimental conditions. By analyzing transcript levels of phosphate and nitrate starvation reporter genes, we confirmed that MpAPT and MpACT are suitable reference genes in M. polymorpha and also demonstrated that normalization with an inappropriate gene can lead to erroneous analysis of qPCR data.
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