Evidence map›Paper›PMID 25798313›Full record

ArticleBiomedical optics express2015

In vivo two-photon imaging of mouse hippocampal neurons in dentate gyrus using a light source based on a high-peak power gain-switched laser diode.

Ryosuke Kawakami, Kazuaki Sawada, Yuta Kusama, Yi-Cheng Fang, Shinya Kanazawa, Yuichi Kozawa, Shunichi Sato, Hiroyuki Yokoyama, Tomomi Nemoto

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
17.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 91 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ryosuke KawakamiResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; Graduate school of information science and technology, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan ; These authors contributed equally to this work.
Kazuaki SawadaResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; Graduate school of information science and technology, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; These authors contributed equally to this work.
Yuta KusamaNew Industry Creation Hatchery Center (NICHe), Tohoku University, 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai, 980-8579, Japan.
Yi-Cheng FangNew Industry Creation Hatchery Center (NICHe), Tohoku University, 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai, 980-8579, Japan.
Shinya KanazawaInstitute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1,Aoba-ku, Sendai, 980-8577, Japan.
Yuichi KozawaInstitute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1,Aoba-ku, Sendai, 980-8577, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan.
Shunichi SatoInstitute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1,Aoba-ku, Sendai, 980-8577, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan.
Hiroyuki YokoyamaNew Industry Creation Hatchery Center (NICHe), Tohoku University, 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai, 980-8579, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan.
Tomomi NemotoResearch Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; Graduate school of information science and technology, Hokkaido University, Kita 20 Nishi 10, Kita-ku, Sapporo, 001-0020, Japan ; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo, Japan.
Tohoku University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In vivo two-photon microscopy is an advantageous technique for observing the mouse brain at high resolution. In this study, we developed a two-photon microscopy method that uses a 1064-nm gain-switched laser diode-based light source with average power above 4 W, pulse width of 7.5-picosecond, repetition rate of 10-MHz, and a high-sensitivity photomultiplier tube. Using this newly developed two-photon microscope for in vivo imaging, we were able to successfully image hippocampal neurons in the dentate gyrus and obtain panoramic views of CA1 pyramidal neurons and cerebral cortex, regardless of age of the mouse. Fine dendrites in hippocampal CA1 could be imaged with a high peak-signal-to-background ratio that could not be achieved by titanium sapphire laser excitation. Finally, our system achieved multicolor imaging with neurons and blood vessels in the hippocampal region in vivo. These results indicate that our two-photon microscopy system is suitable for investigations of various neural functions, including the morphological changes undergone by neurons during physiological phenomena.

Indexed as

(110.6880) Three-dimensional image acquisition(180.0180) Microscopy(180.2520) Fluorescence microscopy(180.4315) Nonlinear microscopy

Identifiers

PMID25798313
PMCPMC4361443
OpenAlexW2046754122

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.