ArticlePlant methods2015
Automated integrative high-throughput phenotyping of plant shoots: a case study of the cold-tolerance of pea (Pisum sativum L.).
Article in Plant methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 92 citations in OpenAlex.
- Tropical vulnerability and temperate resilience: molecular and physiological insights into cold stress adaptation in oil palm.GM crops & food · 2026Review
- A panomics-driven framework for the improvement of major food legume crops: advances, challenges, and future prospects.Horticulture research · 2025Article
- Omics-Driven Strategies for Developing Saline-Smart Lentils: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Implementation of theoretical non-photochemical quenching (NPQScientific reports · 2024Article
- Frost tolerance improvement in pea and white lupin by a high-throughput phenotyping platform.Frontiers in plant science · 2024Article
- Engaging Precision Phenotyping to Scrutinize Vegetative Drought Tolerance and Recovery in Chickpea Plant Genetic Resources.Plants (Basel, Switzerland) · 2023Article
- Genome-wide association study as a powerful tool for dissecting competitive traits in legumes.Frontiers in plant science · 2023Review
- Article
- Applicability of hyperspectral imaging during salinity stress in rice for tracking Na+ and K+ levels in planta.PloS one · 2022Article
- Adaptation of Interspecific Mesoamerican Common Bean Lines to Acid Soils and High Temperature in the Amazon Region of Colombia.Plants (Basel, Switzerland) · 2021Article
- Review
- Omics resources and omics-enabled approaches for achieving high productivity and improved quality in pea (Pisum sativum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Breeding for Climate Change Resilience: A Case Study of Loblolly Pine (Frontiers in plant science · 2021Review
- Dynamics of Maize Vegetative Growth and Drought Adaptability Using Image-Based Phenotyping Under Controlled Conditions.Frontiers in plant science · 2021Article
- Non-destructive phenotyping for early seedling vigor in direct-seeded rice.Plant methods · 2020Article
- Low nitrogen stress regulates chlorophyll fluorescence in coordination with photosynthesis and Rubisco efficiency of rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Low temperature induced modulation of photosynthetic induction in non-acclimated and cold-acclimated Arabidopsis thaliana: chlorophyll a fluorescence and gas-exchange measurements.Photosynthesis research · 2019Article
- CropSight: a scalable and open-source information management system for distributed plant phenotyping and IoT-based crop management.GigaScience · 2019Article
- Image-Derived Traits Related to Mid-Season Growth Performance of Maize Under Nitrogen and Water Stress.Frontiers in plant science · 2019Article
- A Combined Phenotypic and Metabolomic Approach for Elucidating the Biostimulant Action of a Plant-Derived Protein Hydrolysate on Tomato Grown Under Limited Water Availability.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecently emerging approaches to high-throughput plant phenotyping have discovered their importance as tools in unravelling the complex questions of plant growth, development and response to the environment, both in basic and applied science. High-throughput methods have been also used to study plant responses to various types of biotic and abiotic stresses (drought, heat, salinity, nutrient-starving, UV light) but only rarely to cold tolerance.
resultsWe present here an experimental procedure of integrative high-throughput in-house phenotyping of plant shoots employing automated simultaneous analyses of shoot biomass and photosystem II efficiency to study the cold tolerance of pea (Pisum sativum L.). For this purpose, we developed new software for automatic RGB image analysis, evaluated various parameters of chlorophyll fluorescence obtained from kinetic chlorophyll fluorescence imaging, and performed an experiment in which the growth and photosynthetic activity of two different pea cultivars were followed during cold acclimation. The data obtained from the automated RGB imaging were validated through correlation of pixel based shoot area with measurement of the shoot fresh weight. Further, data obtained from automated chlorophyll fluorescence imaging analysis were compared with chlorophyll fluorescence parameters measured by a non-imaging chlorophyll fluorometer. In both cases, high correlation was obtained, confirming the reliability of the procedure described.
conclusionsThis study of the response of two pea cultivars to cold stress confirmed that our procedure may have important application, not only for selection of cold-sensitive/tolerant varieties of pea, but also for studies of plant cold-response strategies in general. The approach, provides a very broad tool for the morphological and physiological selection of parameters which correspond to shoot growth and the efficiency of photosystem II, and is thus applicable in studies of various plant species and crops.
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