ArticleJournal of virological methods2015
A novel method for the measurement of hepatitis C virus infectious titres using the IncuCyte ZOOM and its application to antiviral screening.
Article in Journal of virological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Article
- A Cell State Monitoring System with Integrated In Situ Imaging and pH Detection.Sensors (Basel, Switzerland) · 2023Article
- NS5A domain I antagonises PKR to facilitate the assembly of infectious hepatitis C virus particles.PLoS pathogens · 2023Article
- Current Landscape of Methods to Evaluate Antimicrobial Activity of Natural Extracts.Molecules (Basel, Switzerland) · 2023Review
- Determining the Importance of Peroxisomal Proteins for Viral Infections in Cultured Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach.PloS one · 2021Article
- Article
- Identification of potassium and calcium channel inhibitors as modulators of polyomavirus endosomal trafficking.Antiviral research · 2020Article
- Glibenclamide inhibits BK polyomavirus infection in kidney cells through CFTR blockade.Antiviral research · 2020Article
- Article
- Modulation of calcium signaling pathway by hepatitis C virus core protein stimulates NLRP3 inflammasome activation.PLoS pathogens · 2019Article
- Remodeling of lumbar motor circuitry remote to a thoracic spinal cord injury promotes locomotor recovery.eLife · 2018Article
- Visualisation and analysis of hepatitis C virus non-structural proteins using super-resolution microscopy.Scientific reports · 2018Article
- Agnoprotein Is an Essential Egress Factor during BK Polyomavirus Infection.International journal of molecular sciences · 2018Article
- A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Regulation of hepatitis C virus replication via threonine phosphorylation of the NS5A protein.The Journal of general virology · 2018Article
- A role for domain I of the hepatitis C virus NS5A protein in virus assembly.PLoS pathogens · 2018Article
- AAV-KLF7 Promotes Descending Propriospinal Neuron Axonal Plasticity after Spinal Cord Injury.Neural plasticity · 2017Article
- Foot-and-mouth disease virus genome replication is unaffected by inhibition of type III phosphatidylinositol-4-kinases.The Journal of general virology · 2016Article
- Release of Infectious Hepatitis C Virus from Huh7 Cells Occurs via a trans-Golgi Network-to-Endosome Pathway Independent of Very-Low-Density Lipoprotein Secretion.Journal of virology · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Hepatitis C virus (HCV) is a significant human pathogen infecting 3% of the world population. An infectious molecular clone capable of replicating and releasing infectious virions in cell culture has only been available since 2005, leaving a significant knowledge gap concerning post-RNA replication events such as particle assembly, trafficking and release. Thus, a fast, efficient and accurate method of measuring infectious viral titres is highly desirable. Current methods rely upon manual counting of infected cell foci and so are both labour-intensive and susceptible to human error. Here, we report a novel protocol, which utilises the IncuCyte ZOOM instrument and related software to accurately count infected cells and extrapolation of this data to produce an infectious titre, reported as infectious units per millilitre (IU/mL). This method reduces cost, time and error in experiments. We also demonstrate that this approach is amenable to high-throughput compound screening, thereby expediting the identification of novel antivirals.
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Registered trials
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