ArticleJournal of experimental botany2015
Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 78 citations in OpenAlex.
- Pro-Vitamin A Biofortified Cavendish Banana: Trait Stability in the Field.Plant biotechnology journal · 2026Article
- Identification of Target Genes of Transcription Factor DpAP2 Related to Carotenoid Biosynthesis from Dunaliella Parva.Applied biochemistry and biotechnology · 2026Article
- Mediated pleiotropy drives the negative correlation of total carotenoid and dry matter contents in cassava (Manihot esculenta).Genetics · 2025Article
- Multi-Omics Methods Applied to Flower Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biofortification: an approach to eradicate micronutrient deficiency.Frontiers in nutrition · 2023Review
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing ofFrontiers in plant science · 2022Article
- The utility of metabolomics as a tool to inform maize biology.Plant communications · 2021Review
- Systems biology for crop improvement.The plant genome · 2021Review
- Nitrogen inputs influence vegetative metabolism in maize engineered with a seed-specific carotenoid pathway.Plant cell reports · 2021Article
- Toward Integrated Multi-Omics Intervention: Rice Trait Improvement and Stress Management.Frontiers in plant science · 2021Review
- Biofortification of Cereals and Pulses Using New Breeding Techniques: Current and Future Perspectives.Frontiers in nutrition · 2021Review
- Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In-depth Understanding ofInternational journal of molecular sciences · 2020Article
- Sugar Signaling During Fruit Ripening.Frontiers in plant science · 2020Review
- Microbial-assisted and genomic-assisted breeding: a two way approach for the improvement of nutritional quality traits in agricultural crops.3 Biotech · 2020Review
- Changing Form and Function through Carotenoids and Synthetic Biology.Plant physiology · 2019Article
- Comparative transcriptomics approach in elucidation of carotenoid biosynthesis regulation in grains of rice (Oryza sativa L.).Scientific reports · 2019Article
- Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment.RSC advances · 2019Article
- Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds.Journal of experimental botany · 2018Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm and how global seed metabolism adapted to this expanded biosynthetic capacity. Although enhancement of carotenoid biosynthesis was targeted to the endosperm of maize kernels, a concurrent up-regulation of sterol and fatty acid biosynthesis in the embryo was measured. Targeted terpenoid analysis, and non-targeted metabolomic, proteomic, and transcriptomic profiling revealed changes especially in carbohydrate metabolism in the transgenic line. In-depth analysis of the data, including changes of metabolite pools and increased enzyme and transcript concentrations, gave a first insight into the metabolic variation precipitated by the higher up-stream metabolite demand by the extended biosynthesis capacities for terpenoids and fatty acids. An integrative model is put forward to explain the metabolic regulation for the increased provision of terpenoid and fatty acid precursors, particularly glyceraldehyde 3-phosphate and pyruvate or acetyl-CoA from imported fructose and glucose. The model was supported by higher activities of fructokinase, glucose 6-phosphate isomerase, and fructose 1,6-bisphosphate aldolase indicating a higher flux through the glycolytic pathway. Although pyruvate and acetyl-CoA utilization was higher in the engineered line, pyruvate kinase activity was lower. A sufficient provision of both metabolites may be supported by a by-pass in a reaction sequence involving phosphoenolpyruvate carboxylase, malate dehydrogenase, and malic enzyme.
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