ArticleApplied and environmental microbiology2015
In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Regulation of Type VI Secretion Systems of Burkholderia during transition to intracellular lifestyle.EMBO reports · 2026Article
- Genetic regulation of biosynthesis and resistance to redox-active phenazines inMicrobiology spectrum · 2026Article
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- Symbiotic T6SS affects horizontal transmission ofISME communications · 2025Article
- The patatin-like protein PlpD forms structurally dynamic homodimers in the Pseudomonas aeruginosa outer membrane.Nature communications · 2024Article
- Genomic editing inApplied and environmental microbiology · 2024Article
- Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- Subcellular localization of Type VI secretion system assembly in response to cell-cell contact.The EMBO journal · 2022Article
- Elevated intracellular cyclic-di-GMP level in Shewanella oneidensis increases expression of c-type cytochromes.Microbial biotechnology · 2020Article
- One gene, multiple ecological strategies: A biofilm regulator is a capacitor for sustainable diversity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Pleiotropic Effects of c-di-GMP Content inApplied and environmental microbiology · 2019Article
- Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.PLoS biology · 2019Article
- Key Players and Individualists of Cyclic-di-GMP Signaling inFrontiers in microbiology · 2018Article
- Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.MicrobiologyOpen · 2017Article
- Genes Required for the Anti-fungal Activity of a Bacterial Endophyte Isolated from a Corn Landrace Grown Continuously by Subsistence Farmers Since 1000 BC.Frontiers in microbiology · 2016Article
- Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange.Nature protocols · 2015Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia is an emerging opportunistic pathogen causing life-threatening infections in immunocompromised individuals and in patients with cystic fibrosis, which are often difficult, if not impossible, to treat. Understanding the genetic basis of virulence in this emerging pathogen is important for the development of novel treatment regimes. Generation of deletion mutations in genes predicted to encode virulence determinants is fundamental to investigating the mechanisms of pathogenesis. However, there is a lack of appropriate selectable and counterselectable markers for use in B. cenocepacia, making its genetic manipulation problematic. Here we describe a Gateway-compatible allelic exchange system based on the counterselectable pheS gene and the I-SceI homing endonuclease. This system provides efficiency in cloning homology regions of target genes and allows the generation of precise and unmarked gene deletions in B. cenocepacia. As a proof of concept, we demonstrate its utility by deleting the Bcam1349 gene, encoding a cyclic di-GMP (c-di-GMP)-responsive regulator protein important for biofilm formation.
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