ArticleRNA (New York, N.Y.)2015
Both the autophagy and proteasomal pathways facilitate the Ubp3p-dependent depletion of a subset of translation and RNA turnover factors during nitrogen starvation in Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Med13 is required for efficient P-body recruitment and autophagic degradation of Edc3 following nitrogen starvation.Molecular biology of the cell · 2024Article
- Integrated transcriptomics and metabolomics analyses provide new insights into cassava in response to nitrogen deficiency.Frontiers in plant science · 2024Article
- The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition.The EMBO journal · 2023Article
- A synthetic genetic array screen for interactions with the RNA helicase DED1 during cell stress in budding yeast.G3 (Bethesda, Md.) · 2023Article
- Fission Yeast TORC1 Promotes Cell Proliferation through Sfp1, a Transcription Factor Involved in Ribosome Biogenesis.Molecular and cellular biology · 2023Article
- Deubiquitinase Ubp3 regulates ribophagy and deubiquitinates Smo1 for appressorium-mediated infection by Magnaporthe oryzae.Molecular plant pathology · 2022Article
- The RNA Helicase Ded1 Regulates Translation and Granule Formation during Multiple Phases of Cellular Stress Responses.Molecular and cellular biology · 2022Article
- Translational control by helicases during cellular stress.Methods in enzymology · 2022Article
- Proteaphagy is specifically regulated and requires factors dispensable for general autophagy.The Journal of biological chemistry · 2022Article
- Application of Metabolomics in the Study of Starvation-Induced Autophagy inJournal of fungi (Basel, Switzerland) · 2021Article
- Integration of the metabolome and transcriptome reveals the mechanism of resistance to low nitrogen supply in wild bermudagrass (Cynodon dactylon (L.) Pers.) roots.BMC plant biology · 2021Article
- The structure and function of deubiquitinases: lessons from budding yeast.Open biology · 2020Review
- Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis.Nature plants · 2019Article
- The DEAD-box RNA helicase Ded1 has a role in the translational response to TORC1 inhibition.Molecular biology of the cell · 2019Article
- On the edge of degradation: Autophagy regulation by RNA decay.Wiley interdisciplinary reviews. RNA · 2019Review
- Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Activation of autophagy rescues synaptic and cognitive deficits in fragile X mice.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Functions of unconventional mammalian translational GTPases GTPBP1 and GTPBP2.Genes & development · 2018Article
- Interdependence of Primary Metabolism and Xenobiotic Mitigation Characterizes the Proteome of Bjerkandera adusta during Wood Decomposition.Applied and environmental microbiology · 2018Article
- Molecular definitions of autophagy and related processes.The EMBO journal · 2017Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Protein turnover is an important regulatory mechanism that facilitates cellular adaptation to changing environmental conditions. Previous studies have shown that ribosome abundance is reduced during nitrogen starvation by a selective autophagy mechanism termed ribophagy, which is dependent upon the deubiquitinase Ubp3p. In this study, we asked whether the abundance of various translation and RNA turnover factors are reduced following the onset of nitrogen starvation in Saccharomyces cerevisiae. We found distinct differences in the abundance of the proteins tested following nitrogen starvation: (1) The level of some did not change; (2) others were reduced with kinetics similar to ribophagy, and (3) a few proteins were rapidly depleted. Furthermore, different pathways differentially degraded the various proteins upon nitrogen starvation. The translation factors eRF3 and eIF4GI, and the decapping enhancer Pat1p, required an intact autophagy pathway for their depletion. In contrast, the deadenylase subunit Pop2p and the decapping enzyme Dcp2p were rapidly depleted by a proteasome-dependent mechanism. The proteasome-dependent depletion of Dcp2p and Pop2p was also induced by rapamycin, suggesting that the TOR1 pathway influences this pathway. Like ribophagy, depletion of eIF4GI, eRF3, Dcp2p, and Pop2p was dependent upon Ubp3p to varying extents. Together, our results suggest that the autophagy and proteasomal pathways degrade distinct translation and RNA turnover factors in a Ubp3p-dependent manner during nitrogen starvation. While ribophagy is thought to mediate the reutilization of scarce resources during nutrient limitation, our results suggest that the selective degradation of specific proteins could also facilitate a broader reprogramming of the post-transcriptional control of gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.