ArticlePloS one2015
Calcium alginate gels as stem cell matrix-making paracrine stem cell activity available for enhanced healing after surgery.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- High-Resolution Atomic Force Microscopy Investigation of Alginate Hydrogel Materials in Aqueous Media.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Continuous Production of Highly Functional Vascularized Hepatobiliary Organoids from Human Pluripotent Stem Cells using a Scalable Microfluidic Platform.Advanced functional materials · 2023Article
- Cell Secretome Strategies for Controlled Drug Delivery and Wound-Healing Applications.Polymers · 2022Review
- Shaping and Patterning Supramolecular Materials─Stem Cell-Compatible Dual-Network Hybrid Gels Loaded with Silver Nanoparticles.ACS biomaterials science & engineering · 2022Article
- A comparative study of mesenchymal stem cells cultured as cell-only aggregates and in encapsulated hydrogels.Journal of tissue engineering and regenerative medicine · 2022Article
- A Review on the Adaption of Alginate-Gelatin Hydrogels for 3D Cultures and Bioprinting.Materials (Basel, Switzerland) · 2021Review
- Stem Cell Therapy for Burns: Story so Far.Biologics : targets & therapy · 2021Review
- Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis.Cell reports · 2020Article
- Hydrogel scaffolds with elasticity-mimicking embryonic substrates promote cardiac cellular network formation.Progress in biomaterials · 2020Article
- Microfluidic fabrication of stable collagen microgels with aligned microstructure using flow-driven co-deposition and ionic gelation.Journal of micromechanics and microengineering : structures, devices, and systems · 2020Article
- An Alginate-Based Hydrogel with a High Angiogenic Capacity and a High Osteogenic Potential.BioResearch open access · 2020Article
- Assaying How Phagocytic Success Depends on the Elasticity of a Large Target Structure.Biophysical journal · 2019Article
- Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings.Marine drugs · 2019Article
- Dual Crosslinked Methacrylated Alginate Hydrogel Micron Fibers and Tissue Constructs for Cell Biology.Marine drugs · 2019Article
- Sustained Release of Basic Fibroblast Growth Factor (bFGF) Encapsulated Polycaprolactone (PCL) Microspheres Promote Angiogenesis In Vivo.Nanomaterials (Basel, Switzerland) · 2019Article
- 3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches.Bioengineering (Basel, Switzerland) · 2019Article
- Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion.Bioengineering (Basel, Switzerland) · 2018Article
- Stem Cells and Engineered Scaffolds for Regenerative Wound Healing.Bioengineering (Basel, Switzerland) · 2018Review
- In situ forming injectable hydrogels for drug delivery and wound repair.Advanced drug delivery reviews · 2018Review
- A New High Throughput Screening Platform for Cell Encapsulation in Alginate Hydrogel Shows Improved Hepatocyte Functions by Mesenchymal Stromal Cells Co-encapsulation.Frontiers in medicine · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regeneration after surgery can be improved by the administration of anabolic growth factors. However, to locally maintain these factors at the site of regeneration is problematic. The aim of this study was to develop a matrix system containing human mesenchymal stem cells (MSCs) which can be applied to the surgical site and allows the secretion of endogenous healing factors from the cells. Calcium alginate gels were prepared by a combination of internal and external gelation. The gelling behaviour, mechanical stability, surface adhesive properties and injectability of the gels were investigated. The permeability of the gels for growth factors was analysed using bovine serum albumin and lysozyme as model proteins. Human MSCs were isolated, cultivated and seeded into the alginate gels. Cell viability was determined by AlamarBlue assay and fluorescence microscopy. The release of human VEGF and bFGF from the cells was determined using an enzyme-linked immunoassay. Gels with sufficient mechanical properties were prepared which remained injectable through a syringe and solidified in a sufficient time frame after application. Surface adhesion was improved by the addition of polyethylene glycol 300,000 and hyaluronic acid. Humans MSCs remained viable for the duration of 6 weeks within the gels. Human VEGF and bFGF was found in quantifiable concentrations in cell culture supernatants of gels loaded with MSCs and incubated for a period of 6 weeks. This work shows that calcium alginate gels can function as immobilization matrices for human MSCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.