ArticlePloS one2015
Identification and molecular characterization of the homogentisate pathway responsible for pyomelanin production, the major melanin constituents in Aeromonas media WS.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Strain-Dependent Thermoadaptation in the Fish PathogenMicroorganisms · 2025Article
- Pyomelanin-powered whole-cell biosensor for ultrasensitive and selective detection of bioavailable Hg(ii).RSC advances · 2025Article
- Investigation of Eumelanin Biosynthesis inMicroorganisms · 2025Article
- From detection to remediation: the potential of phosphomelanin in sustainable environmental solutions.Frontiers in bioengineering and biotechnology · 2025Article
- Mutation ofMicrobiology spectrum · 2024Article
- Bioproduction and optimization of newly characterized melanin pigment from Streptomyces djakartensis NSS-3 with its anticancer, antimicrobial, and radioprotective properties.Microbial cell factories · 2024Article
- Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi.Microbial cell factories · 2022Article
- Comparative Genomics of Typical and AtypicalMicroorganisms · 2022Article
- Melanin Produced bymSphere · 2021Article
- Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide.Scientific reports · 2021Article
- Melanins as Sustainable Resources for Advanced Biotechnological Applications.Global challenges (Hoboken, NJ) · 2021Review
- Draft Genome Sequences and Genomic Analysis for Pigment Production in Bacteria Isolated from Blue Discolored Soymilk and Tofu.Journal of genomics · 2021Article
- Article
- Pyomelanin-ProducingFrontiers in microbiology · 2021Article
- Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-algaF1000Research · 2020Article
- A robust bacterial assay for high-throughput screening of human 4-hydroxyphenylpyruvate dioxygenase inhibitors.Scientific reports · 2019Article
- From Extraction to Advanced Analytical Methods: The Challenges of Melanin Analysis.International journal of molecular sciences · 2019Review
- 4-Hydroxyphenylpyruvate Dioxygenase Thermolability Is Responsible for Temperature-Dependent Melanogenesis in Aeromonas salmonicida subsp.Applied and environmental microbiology · 2019Article
- Single Amino Acid Substitution in Homogentisate Dioxygenase Affects Melanin Production inFrontiers in microbiology · 2018Article
- Pyomelanin from Pseudoalteromonas lipolytica reduces biofouling.Microbial biotechnology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pigmentation of many Aeromonas species has been thought to be due to the production of a L-DOPA (L-3,4-dihydroxyphenylalanine) based melanin. However, in this study we found that although L-DOPA synthesis occurs in the high-melanin-yielding Aeromonas media strain WS, it plays a minor, if any, role in pigmentation. Instead, the pigmentation of A. media strain WS is due to the production of pyomelanin through HGA (homogentisate). Gene products of phhA (encodes phenylalanine hydroxylase), tyrB and aspC (both encode aromatic amino acid aminotransferase), and hppD (encodes 4-hydroxyphenylpyruvate dioxygenase) constitute a linear pathway of converting phenylalanine to HGA and disruption of any one of these genes impairs or blocks pigmentation of A. media strain WS. This HGA biosynthesis pathway is widely distributed in Aeromonas, but HGA is only detectable in the cultures of pigmented Aeromonas species. Heterologous expression of HppD from both pigmented and non-pigmented Aeromonas species in E. coli leads to the production of pyomelanin and thus pigmentation, suggesting that most Aeromonas species have the critical enzymes to produce pyomelanin through HGA. Taken together, we have identified a widely conserved biosynthesis pathway of HGA based pyomelanin in Aeromonas that may be responsible for pigmentation of many Aeromonas species.
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