ArticlePloS one2015
Microporous dermal-mimetic electrospun scaffolds pre-seeded with fibroblasts promote tissue regeneration in full-thickness skin wounds.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- 3D hierarchically aligned nanofiber scaffolds promote cell migration for tissue regeneration.Nature protocols · 2026Review
- Exploring New Horizons in Wound Healing: A Comprehensive Analysis.Recent advances in inflammation & allergy drug discovery · 2026Review
- Dual delivery gene-activated scaffold directs fibroblast activity and keratinocyte epithelization.APL bioengineering · 2024Article
- Molecular-scale substrate anisotropy, crowding and division drive collective behaviours in cell monolayers.Journal of the Royal Society, Interface · 2023Article
- Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing.Polymers · 2021Article
- Induced Periosteum-Mimicking Membrane with Cell Barrier and Multipotential Stromal Cell (MSC) Homing Functionalities.International journal of molecular sciences · 2020Article
- New forms of electrospun nanofiber materials for biomedical applications.Journal of materials chemistry. B · 2020Review
- Strategies to Improve Nanofibrous Scaffolds for Vascular Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2020Review
- Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems.Antibiotics (Basel, Switzerland) · 2020Review
- Toward Biomimetic Scaffolds for Tissue Engineering: 3D Printing Techniques in Regenerative Medicine.Frontiers in bioengineering and biotechnology · 2020Review
- 3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds.Biomaterials research · 2019Article
- [Experimental study on long-term outcome of porcine collagen membrane xenotransplantationZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Article
- Emerging Roles of Electrospun Nanofibers in Cancer Research.Advanced healthcare materials · 2018Review
- Biomaterials for Skin Substitutes.Advanced healthcare materials · 2018Review
- Skin Tissue Substitutes and Biomaterial Risk Assessment and Testing.Frontiers in bioengineering and biotechnology · 2018Review
- A Novel Silver Bioactive Glass Elicits Antimicrobial Efficacy AgainstFrontiers in microbiology · 2018Article
- Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering.Tissue engineering and regenerative medicine · 2017Review
- Recent advances in electrospun nanofibers for wound healing.Nanomedicine (London, England) · 2017Review
- Article
- Endoscopic Closure for Full-Thickness Gastrointestinal Defects: Available Applications and Emerging Innovations.Clinical endoscopy · 2016Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Electrospun scaffolds serve as promising substrates for tissue repair due to their nanofibrous architecture and amenability to tailoring of chemical composition. In this study, the regenerative potential of a microporous electrospun scaffold pre-seeded with dermal fibroblasts was evaluated. Previously we reported that a 70% collagen I and 30% poly(Ɛ-caprolactone) electrospun scaffold (70:30 col/PCL) containing 160 μm diameter pores had favorable mechanical properties, supported fibroblast infiltration and subsequent cell-mediated deposition of extracellular matrix (ECM), and promoted more rapid and effective in vivo skin regeneration when compared to scaffolds lacking micropores. In the current study we tested the hypothesis that the efficacy of the 70:30 col/PCL microporous scaffolds could be further enhanced by seeding scaffolds with dermal fibroblasts prior to implantation into skin wounds. To address this hypothesis, a Fischer 344 (F344) rat syngeneic model was employed. In vitro studies showed that dermal fibroblasts isolated from F344 rat skin were able to adhere and proliferate on 70:30 col/PCL microporous scaffolds, and the cells also filled the 160 μm pores with native ECM proteins such as collagen I and fibronectin. Additionally, scaffolds seeded with F344 fibroblasts exhibited a low rate of contraction (~14%) over a 21 day time frame. To assess regenerative potential, scaffolds with or without seeded F344 dermal fibroblasts were implanted into full thickness, critical size defects created in F344 hosts. Specifically, we compared: microporous scaffolds containing fibroblasts seeded for 4 days; scaffolds containing fibroblasts seeded for only 1 day; acellular microporous scaffolds; and a sham wound (no scaffold). Scaffolds containing fibroblasts seeded for 4 days had the best response of all treatment groups with respect to accelerated wound healing, a more normal-appearing dermal matrix structure, and hair follicle regeneration. Collectively these results suggest that microporous electrospun scaffolds pre-seeded with fibroblasts promote greater wound-healing than acellular scaffolds.
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