ArticlePloS one2015
Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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37 citing papers in PubMed, 69 citations in OpenAlex.
- Auxin-related regulation of barley floral organ fate: genetic and phenotypic insights from tweaky spike mutants.The New phytologist · 2026Article
- Comparative transcriptome analysis between salt-tolerant and salt-sensitive naked barley landraces under salt stress at the germination stage.BMC plant biology · 2025Article
- Early Seedling Screening Reveals Unidentified Al Resistance Mechanisms in Lithuanian Barley Cultivars.International journal of molecular sciences · 2025Article
- Validation of suitable reference microRNAs for qRT-PCR inFrontiers in plant science · 2025Article
- Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics.Metabolites · 2023Article
- Comparative transcriptome analysis reveals molecular regulation of salt tolerance in two contrasting chickpea genotypes.Frontiers in plant science · 2023Article
- MicroRNAs expression profiles in early responses to different levels of water deficit inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Lactic Acid Bacteria as Potential Biocontrol Agents for Fusarium Head Blight Disease of Spring Barley.Frontiers in microbiology · 2022Article
- Expression of Specific Alleles of Zinc-Finger Transcription Factors,International journal of molecular sciences · 2021Article
- Regulation of the plastochron by three many-noded dwarf genes in barley.PLoS genetics · 2021Article
- Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan.BMC plant biology · 2020Article
- Regulation of Sulfate Uptake and Assimilation in Barley (Plants (Basel, Switzerland) · 2020Article
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- Analysis of Small RNAs of Barley Genotypes Associated with Resistance to Barley Yellow Dwarf Virus.Plants (Basel, Switzerland) · 2020Article
- The Impact of Zinc Oxide Nanoparticles on Cytotoxicity, Genotoxicity, and miRNA Expression in Barley (TheScientificWorldJournal · 2020Article
- The BarleyFrontiers in genetics · 2020Article
- Biosynthesis of Phenylamide Phytoalexins in Pathogen-Infected Barley.International journal of molecular sciences · 2019Article
- Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants.Nature communications · 2019Article
- Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For accurate and reliable gene expression analysis using quantitative real-time reverse transcription PCR (qPCR), the selection of appropriate reference genes as an internal control for normalization is crucial. We hypothesized that non-coding, small nucleolar RNAs (snoRNAs)would be stably expressed in different barley varieties and under different experimental treatments,in different tissues and at different developmental stages of plant growth and therefore might prove to be suitable reference genes for expression analysis of both microRNAs (miRNAs)and mRNAs. In this study, we examined the expression stability of ten candidate reference genes in six barley genotypes under five experimental stresses, drought, fungal infection,boron toxicity, nutrient deficiency and salinity. We compared four commonly used housekeeping genes; Actin (ACT), alpha-Tubulin (α-TUB), Glycolytic glyceraldehyde-3-phosphate dehydrogenase(GAPDH), ADP-ribosylation factor 1-like protein (ADP), four snoRNAs; (U18,U61, snoR14 and snoR23) and two microRNAs (miR168, miR159) as candidate reference genes. We found that ADP, snoR14 and snoR23 were ranked as the best of these candidates across diverse samples. Additionally, we found that miR168 was a suitable reference gene for expression analysis in barley. Finally, we validated the performance of our stable and unstable candidate reference genes for both mRNA and miRNA qPCR data normalization under different stress conditions and demonstrated the superiority of the stable candidates. Our data demonstrate the suitability of barley snoRNAs and miRNAs as potential reference genes form iRNA and mRNA qPCR data normalization under different stress treatments [corrected].
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