ArticlePLoS neglected tropical diseases2015
Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Biological function of Extracellular Vesicles (EVs): a review of the field.Molecular biology reports · 2023Review
- Recent Advances on the Function and Purification of Milk Exosomes: A Review.Frontiers in nutrition · 2022Review
- Antigen-Specific Urinary Immunoglobulin in Reservoir Hosts of Leptospirosis.Veterinary sciences · 2021Article
- Urinary Exosomes Identify Inflammatory Pathways in Vancomycin Associated Acute Kidney Injury.International journal of molecular sciences · 2021Article
- Investigating the Immunological and Biological Equilibrium of Reservoir Hosts and PathogenicFrontiers in microbiology · 2020Review
- Identification of Maltase Glucoamylase as a Biomarker of Acute Kidney Injury in Patients with Cirrhosis.Critical care research and practice · 2019Article
- Pathology and pathogenesis of human leptospirosis: a commented review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Review
- Exosomes in Pathogen Infections: A Bridge to Deliver Molecules and Link Functions.Frontiers in immunology · 2018Review
- Extracellular Vesicles in Renal Pathophysiology.Frontiers in molecular biosciences · 2017Review
- Proteomic analyses of Urine Exosomes reveal New Biomarkers of Diabetes in Pregnancy.Madridge journal of diabetes · 2016Article
- Correction: Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats.PLoS neglected tropical diseases · 2015Article
Corrections and comments
- Erratum issued
- Erratum issuedCorrection: Correction: Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats.2015 · Mondrogon, Chanthel-Kokoy [corrected to Kokoy-Mondragon, Chanthel]
Authors and funding
19 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundInfectious Leptospira colonize the kidneys of reservoir (e.g. rats) and accidental hosts such as humans. The renal response to persistent leptospiral colonization, as measured by urinary protein biosignatures, has not been systematically studied. Urinary exosomes--bioactive membrane-bound nanovesicles--contain cell-state specific cargo that additively reflect formation all along the nephron. We hypothesized that Leptospira-infection will alter the content of urine exosomes, and further, that these Leptospira-induced alterations will hold clues to unravel novel pathways related to bacterial-host interactions. METHODOLOGY/PRINCIPAL
findingsExosome protein content from 24 hour urine samples of Leptospira-infected rats was compared with that of uninfected rats using SDS-PAGE and liquid chromatography/tandem mass spectrometry (LC-MS/MS). Statistical models were used to identify significantly dysregulated proteins in Leptospira-infected and uninfected rat urine exosomes. In all, 842 proteins were identified by LC-MS/MS proteomics of total rat urine and 204 proteins associated specifically with exosomes. Multivariate analysis showed that 25 proteins significantly discriminated between uninfected control and infected rats. Alanyl (membrane) aminopeptidase, also known as CD13 topped this list with the highest score, a finding we validated by Western immunoblotting. Whole urine analysis showed Tamm-Horsfall protein level reduction in the infected rat urine. Total urine and exosome proteins were significantly different in male vs. female infected rats.
conclusionsWe identified exosome-associated renal tubule-specific responses to Leptospira infection in a rat chronic colonization model. Quantitative differences in infected male and female rat urine exosome proteins vs. uninfected controls suggest that urine exosome analysis identifies important differences in kidney function that may be of clinical and pathological significance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.